AAB HEMATOLOGY questions and answrs(latest upate).

insufficient centrifugation can result in false increase in Hct value Hct def. volume of packed red cells mature erythrocyte is approx. ___ um 7 microns rbc with increased central pallor hypochromic preferable site for bone marrow aspiration iliac crest Mean cell volume (MCV) formula (Hct / RBC) x 10 = µm³ avg volume of red cells is ___ MCV ___is the bluish tinge of an immature erythrocyte (reticulocyte) circulating in peripheral blood polychromasia Mean Cell Hemoglobin Conc (MCHC) formula (Hgb / Hct) x 100 Avg conc. of Hgb in red cells expressed as a percent MCHC (expresses ratio of weight of Hgb to the vol of red cells formula for cell count hemocytometer (# cells x dilution factor x depth factor(10) ) / divided by area (9) = mm³ or x 10^9 (sub. 3 decimal places) rbc's containing iron granules, visible with Prussian blue stain Siderocyte EDTA tube containing low volume of blood will have which test erroneous Hct (too much anticoagulant shrinks cells) rbc count formula #cells counted x dilution factor x depth factor(10) / area phagocytic cell produce lysozymes that are bacteriocidal neutrophils ___are poorly phagocytic and do not release lysozyme eosinophils ___ has to be corrected when for anemia retic count corrected retic count for low hct (anemia) formula retic % x (Hct / 45) decr. osmotic fragility assoc with sickle cell anemia ___ is increased with spherocytes and decreased with sickle cells and target cells osmotic fragility Wright's-stained smear needs buffer at what pH cannot be lower then 6.4 (red cells too pink) RBC inclusion CAN be seen with supravital stain, but NOT with Wright's stain Heinz bodies variance in size of red cell population, calc by electronic cell counters RDW (red cell distribution width) Ocular device used to facilitate counting of platelets miller disk used to stain retics supravital stain Coulter cell counter uses principal of electrical impedence rbc with decreased cell diameter and volume spherocyte describes the distribution of a sample of observations standard deviation organ, pitting process spleen spherocytes differ from normal rbc's by decreased surface to volume, decreased resistance to hypertonic solution, decreased deformability hereditary spherocytosis charactgerized by increased osmotic fragility, MCHC >36, extravascular hemolysis % of Hgb S, Hgb A in sickle cell A, 50-70% S, 20-40% sickle cell amino acid substitution is valine for glutamic acid at sixth position Beta chain found in Hgb C Hgb C crystals, target cells, Lysine subed for