Lecture 2: Expression systems
Protein Expression systems
Therapeutic Proteins
Learning Aims
Review available recombinant DNA and protein technology
Review what vectors are available to carry and express a gene of interest
Discuss what benefits the different expression systems have
Learning Outcomes
Be able to consider and evaluate the questions that should be asked when deciding
on a suitable expression system
Outline the advantages and disadvantages of the following expression systems
1. Prokaryotes (eg., E Coli)
2. Methylotrophic and other yeasts
3. Insects
4. Mammalian cell-cultures
5. Transgenic animal / Transgenic plants
Why protein Expression and purification?
A. Basic scientific applications
B. Therapeutic p roteins
Expression systems
BioPharma industry is dependent on;
recombinant protein expression
high levels of expression
reproducible quality
This will allow;
production of sufficient protein
analysis and better understanding of protein products
research and development of new therapeutics
DNA cloning
, Expression Vector features
Protein Expression systems
Therapeutic Proteins
Learning Aims
Review available recombinant DNA and protein technology
Review what vectors are available to carry and express a gene of interest
Discuss what benefits the different expression systems have
Learning Outcomes
Be able to consider and evaluate the questions that should be asked when deciding
on a suitable expression system
Outline the advantages and disadvantages of the following expression systems
1. Prokaryotes (eg., E Coli)
2. Methylotrophic and other yeasts
3. Insects
4. Mammalian cell-cultures
5. Transgenic animal / Transgenic plants
Why protein Expression and purification?
A. Basic scientific applications
B. Therapeutic p roteins
Expression systems
BioPharma industry is dependent on;
recombinant protein expression
high levels of expression
reproducible quality
This will allow;
production of sufficient protein
analysis and better understanding of protein products
research and development of new therapeutics
DNA cloning
, Expression Vector features
