Histotechnology- Chapter 3 (Instrumentation) questions with answer 2024

Histotechnology- Chapter 3 (Instrumentation)List the equipment in the lab that employs temperature control and state the proper temperature for each instrument. - correct answers Cryostat- -20C (normal), -10C (brain, liver, spleen, lymph node), -25C (Fatty tissue) Flotation Bath- 5-10C Dryer & Oven- 60C Incubator Oven- 37C Fridge- 4C Freezer- -20C Describe light microscopy and identify indications for its use. - correct answers -used for the examination of tissue sections -combines 2 simple microscopes (compass microscope) -image appears dark on a light background Describe Polarizing microscopy and identify indications for its use. - correct answers -primarily used in the identification of crystals or amyloid stained with Congo red -used to examine tissue for substances exhibiting double refraction, an isotropism, and birefringence -achieved by interposing a polarizing device between the light source and the specimen, and inserting a polarizing filter between the specimen and the eye Describe Phase-Contrast microscopy and identify indications for its use. - correct answers -used to examine unstained specimens and allows transparent objects to be seen -not used in routine histology Describe Darkfield microscopy and identify indications for its use. - correct answers -directly transmitted light is excluded, and only scattered or oblique light is used used for study of unstained microorganisms and for silver grains in radioactive staining procedures