CMMB 461 Final Exam Actual Questions and Answers with complete solution

CMMB 461 Final Exam Actual Questions and Answers with complete solution what is the gene silencing process? - answergene silencing is sequence specific and mediated by double stranded RNA molecules (like siRNAs and shRNA/microRNA) at what level is gene silencing? - answerthe post-transcriptional level (mRNA degradation and translation repression) where was gene silencing first initially observed? - answerinitially observed in petunias where over-expression of a pigmentation gene resulted in its own down regulation (also called co-suppression of gene expression) what specifically cause gene silencing? - answerdouble stranded RNA causes gene silencing NOT sense and antisense RNA how do you get small interfering RNAs (siRNAs) - answerlong double stranded RNAs (400- 700 bp) is cleaved to 21-23 bp fragments via Dicer RNAse III to generate siRNAs what does siRNA serve as a function for? - answersiRNAs are unwound and one of the strands functions as a template in RISC to guide cleavage of the complementary mRNA what does RISC stand for? - answerRNA induced-silencing complex