Behaviour of biomolecules: NMR Spectroscopy lecture notes

BIOL1021:
Behavior  of  Biomolecules:
Nuclear  Magnetic  Resonance
Spectroscopy
Dr  Philip  T.F.  Williamson

Centre  for  Biological  Sciences

, ROYAL SOCIETY OF CHEMISTRY DATA BOOK
THE ELECTROMAGNETIC SPECTRUM

The  electromagnetic  spectrum
f / Hz /m
1020 3 x 10­12

1019
X ­ rays
X-­ray 1018
420 nm (4.20 x 10­7 m)
Spectroscopy 1017 3x 10­9
Violet


Far ultraviolet Blue 470 nm
1016
UV/Vis
1015 3 x 10­7 Ultraviolet Green 530 nm
Spectroscopy
1014 Yellow 580 nm
Rotational/Vibrational Infrared
Spectroscopy 1013 3 x 10­5 Orange 620 nm
Rotational Far infrared
1012
Spectroscopy Red
700 nm (7.00 x 10­7 m)
1011 3 x 10­3
Electron  Resonance Microwave
1010 3 x 10­2
(NMR/MRI)
109 3 x 10­1

108 3
Nuclear  Magnetic
Resonance 107 Radio
(NMR/MRI)
106 3 x 102

105 2

, Spin
Spin  (I)  is  an  intrinsic  property  of
some  nuclei  and  electrons.

In  absence  of  magnetic  field
energy  levels  are  degenerate
Apply field

In  presence  of  applied  field  can   B0
adopt  2I+1  energy  levels

For  example:
ΔEmag=-µ.B0
• 1H,   13C,   15N,   31P  are  I=1/2
• 2H,   14N,  are  I=1
• 11B,   23Na,   35Cl  are  I=3/2
3