Microbiology Lab Exam 2 – Questions and Answers (100% Correct)

What three color pencils are needed? - ANSWER - blue, red, green How are endospores freed form the vegetative cell in which they form? - ANSWER - released when losses the surrounding sporangium What drugs are used to treat tuberculosis? - ANSWER - rifampin, isoniazid, ehtambutol, and pyrazinamide What drugs are used to treat leprosy? - ANSWER - rifampin What other disease besides tuberculosis is caused by pathogenic mycobacterium? In what animal to they grow? - ANSWER - leprosy (Hansen's disease); rats and armadillos What specimen is usually obtained to diagnose tuberculosis? - ANSWER - sputum What cultural characteristic of the mycobacteria would indicate that the diseases they cause would probably be long term chronic diseases rather than progressing acute ones? - ANSWER - have a slow bacteria growth What would happen if acid alcohol was used in the Gram stain instead of regular alcohol? - ANSWER - it all would be colorless due to the HCL, and hard to differentia between gram positive and gram negative What makes the mycobacterium stain differently from most other bacteria? - ANSWER - because their cells consist of an outer wall that holds fast/tightly to the dye even when washed with acid alcohol. Made of mycelic acid; waxy part by wall contributes to high resistance Bunsen burner - ANSWER - produces gas flame, used to heat and sterilize inoculating needles and loops Flint lighter - ANSWER - tripod stand - ANSWER - Wire gauze with asbestos center: since it's a good conductor of heat, prevents the beaker from cracking - ANSWER - Inoculating loop - ANSWER - to retrieve an inoculum for streaking and transfer microscopic organisms