Protocols for vitamin c content in fruit
The experiment I will be conducting is testing to see which fruit has the most amount of vitamin c. As a
result, I have listed three potential protocols I'll be using to carry out my experiment.
Hypothesis
In this experiment I’ll be carrying out, I hypothesize that orange juice will contain the most vitamin c.
This is because I have seen on tv and online that orange juice is advertised as a having a lot of vitamin c
and is recommended highly to children to increase their intake.
Null hypothesis
In this experiment there will be no significant difference between the fruit juices being tested.
Protocol 1
1. Add 1cm3 of 1% blue DCPIP into a conical flask.
2. Fill a burette with your first choice of fruit juice and make note of the start value.
3. Drop by drop add the fruit juice using the burette to the DCPIP and swirl the flask whilst the
other hand is on the tap.
4. As soon as the DCPIP loses the blue colour close the tap and note down the value.
5. Calculate the amount of fruit juice needed to decolourise the DCPIP and note it down in a table.
6. Repeat the procedure with the other fruit juices.
https://snabbiology.co.uk/measuring-the-content-of-vitamin-c-in-fruit-juice/
iv- fruit juices
Equipment Chemical
Conical flask DCPIP
Burette
Fruit juice
Protocol 2
Make up a 1% solution of vitamin C with 1 g of vitamin C in 100 cm 3; this is 10 mg cm3.
b Make up a 1% solution of DCPIP.
Investigation
c Pipette 2 cm3 of vitamin C solution into a test tube.
d Using a graduated pipette or a burette, add 1% DCPIP drop by drop to the vitamin C solution.
Shake the tube gently after adding each drop. Add DCPIP solution until the blue colour of the
final drop does not disappear.
e Record the exact amount of DCPIP solution that was added.
The experiment I will be conducting is testing to see which fruit has the most amount of vitamin c. As a
result, I have listed three potential protocols I'll be using to carry out my experiment.
Hypothesis
In this experiment I’ll be carrying out, I hypothesize that orange juice will contain the most vitamin c.
This is because I have seen on tv and online that orange juice is advertised as a having a lot of vitamin c
and is recommended highly to children to increase their intake.
Null hypothesis
In this experiment there will be no significant difference between the fruit juices being tested.
Protocol 1
1. Add 1cm3 of 1% blue DCPIP into a conical flask.
2. Fill a burette with your first choice of fruit juice and make note of the start value.
3. Drop by drop add the fruit juice using the burette to the DCPIP and swirl the flask whilst the
other hand is on the tap.
4. As soon as the DCPIP loses the blue colour close the tap and note down the value.
5. Calculate the amount of fruit juice needed to decolourise the DCPIP and note it down in a table.
6. Repeat the procedure with the other fruit juices.
https://snabbiology.co.uk/measuring-the-content-of-vitamin-c-in-fruit-juice/
iv- fruit juices
Equipment Chemical
Conical flask DCPIP
Burette
Fruit juice
Protocol 2
Make up a 1% solution of vitamin C with 1 g of vitamin C in 100 cm 3; this is 10 mg cm3.
b Make up a 1% solution of DCPIP.
Investigation
c Pipette 2 cm3 of vitamin C solution into a test tube.
d Using a graduated pipette or a burette, add 1% DCPIP drop by drop to the vitamin C solution.
Shake the tube gently after adding each drop. Add DCPIP solution until the blue colour of the
final drop does not disappear.
e Record the exact amount of DCPIP solution that was added.
