Equipment and Reagent Request List
(You will need to request the equipment that you want to use in your investigation)
*= Shared equipment (not stored in individual tray)
Name: Analies Fatima Pires Tray number Group B
(eg MM,SH)
Title of Investigation: To investigate the effect of caffeine & nicotine on heart rate in Daphnia.
Water baths Tick Testing Reagents Tick
Water baths * 5C I/KI: For use with dropping tile (conc.)
Beakers and Ice 15C I/KI: For use with colorimeter (dilute)
Water baths * 25C DCPIP (0.1%) – (use with drinks eg orange juice)
Electric Thermostatically controlled 35C DCPIP (0.02%) – (use with pepper extracts)
45C Benedict’s reagent
55C Potassium Permanganate
70C Sulphuric Acid
Distilled Water (Bottle)* ✓
Water bath (individual: metal)* Testing equipment Tick
(Bunsen Burner, Tripod and Mat also required) Colorimeter*
Spotting Tile ✓
Glassware No Glassware (cont) No Glassware (cont) No
Boiling tube (10) Measuring Cylinder (10ml) Syringe (1ml)
Test Tube (10) Measuring Cylinder (50ml) Syringe (2ml)
Test/Boiling Tube Brush(s)* Measuring Cylinder (100ml) Syringe (5ml)
Test/Boiling Tube Rack* Measuring Cylinder (250ml) Syringe (10ml)
Bung (Test Tube) Graduated pipette (1ml) Pasteur pipette 1
Bung (Boiling Tube) Graduated pipette (5ml)
Small Beaker (50ml) Graduated pipette (10ml)
Funnel Pipette filler
General Equipment General Equipment (cont) General Equipment (cont)
Chinagraph pencil Pestle and Mortar*
Marker Pen* 1 Liquidiser /Blender* 1
Tea Strainer 1 Sticky Labels 4 Electronic Balance* 1
Cork Borer* Glass Rod Microscope*
Knife* 1 Stop clock* Cavity Slide*
Counter* Colorimeter* Daphnia*
Spatula* 1 Cuvette (tube for colorimeter)
Enzymes Tick Substrates Tick “Foods” Tick
✓
Starch:1% Apple
Bacterial Amylase: 1% Marvel (Protein): 2% Lemon 3 ✓
✓
Fungal Amylase: 1% Lime 3
Mammalian Protease (Trypsin):1% Carrot 3 ✓
Bacterial Protease: 1% Potato
Yeast (source of Catalase) Hydrogen Peroxide (100%) Green/Red/Yellow Pepper
Pepsin Beetroot
Trypsin Other Chemicals Drinks
Lead Nitrate: Pb(NO3)2: 1% Apple Juice (fresh/long life/tinned)
(You will need to request the equipment that you want to use in your investigation)
*= Shared equipment (not stored in individual tray)
Name: Analies Fatima Pires Tray number Group B
(eg MM,SH)
Title of Investigation: To investigate the effect of caffeine & nicotine on heart rate in Daphnia.
Water baths Tick Testing Reagents Tick
Water baths * 5C I/KI: For use with dropping tile (conc.)
Beakers and Ice 15C I/KI: For use with colorimeter (dilute)
Water baths * 25C DCPIP (0.1%) – (use with drinks eg orange juice)
Electric Thermostatically controlled 35C DCPIP (0.02%) – (use with pepper extracts)
45C Benedict’s reagent
55C Potassium Permanganate
70C Sulphuric Acid
Distilled Water (Bottle)* ✓
Water bath (individual: metal)* Testing equipment Tick
(Bunsen Burner, Tripod and Mat also required) Colorimeter*
Spotting Tile ✓
Glassware No Glassware (cont) No Glassware (cont) No
Boiling tube (10) Measuring Cylinder (10ml) Syringe (1ml)
Test Tube (10) Measuring Cylinder (50ml) Syringe (2ml)
Test/Boiling Tube Brush(s)* Measuring Cylinder (100ml) Syringe (5ml)
Test/Boiling Tube Rack* Measuring Cylinder (250ml) Syringe (10ml)
Bung (Test Tube) Graduated pipette (1ml) Pasteur pipette 1
Bung (Boiling Tube) Graduated pipette (5ml)
Small Beaker (50ml) Graduated pipette (10ml)
Funnel Pipette filler
General Equipment General Equipment (cont) General Equipment (cont)
Chinagraph pencil Pestle and Mortar*
Marker Pen* 1 Liquidiser /Blender* 1
Tea Strainer 1 Sticky Labels 4 Electronic Balance* 1
Cork Borer* Glass Rod Microscope*
Knife* 1 Stop clock* Cavity Slide*
Counter* Colorimeter* Daphnia*
Spatula* 1 Cuvette (tube for colorimeter)
Enzymes Tick Substrates Tick “Foods” Tick
✓
Starch:1% Apple
Bacterial Amylase: 1% Marvel (Protein): 2% Lemon 3 ✓
✓
Fungal Amylase: 1% Lime 3
Mammalian Protease (Trypsin):1% Carrot 3 ✓
Bacterial Protease: 1% Potato
Yeast (source of Catalase) Hydrogen Peroxide (100%) Green/Red/Yellow Pepper
Pepsin Beetroot
Trypsin Other Chemicals Drinks
Lead Nitrate: Pb(NO3)2: 1% Apple Juice (fresh/long life/tinned)
