Intro to Genetic Disorders
Firstly need a solid
grasp of miniosis
the
Replication
DNA
bits
key to understand:
-Replication is semi conservative -
Each strand is a
template
strand
I each daughter has
halforiginal
& other Enz.
Done
by DNA
Polymerases
-
There is a
leading stand s lagging strand due to
51+ 3' direction
replication in
process:
the
Helicase breaks H-bonds
-DNA bowl
bases separates
-
I strands
↳ stands coated w/
single-strand binding proteins
strands
rejoining early
-
to
stop
-Primase constructs a
primer ofRNAcomplementary to template,
which gives polymerase something bind to
DNA to
DNA
Polymerase adds nucleotides
direction
to exposed bases
in a
semi
↳ Easily done in one go for leading stand
↳
lagging strand is the other way so is
in small sections which then
formed are
joined
DNA
polymerase also PROOF-READS for errors
↳ Forms backbone on own
↳ Fragments oflagging side joined together by
DNA
ligase
Firstly need a solid
grasp of miniosis
the
Replication
DNA
bits
key to understand:
-Replication is semi conservative -
Each strand is a
template
strand
I each daughter has
halforiginal
& other Enz.
Done
by DNA
Polymerases
-
There is a
leading stand s lagging strand due to
51+ 3' direction
replication in
process:
the
Helicase breaks H-bonds
-DNA bowl
bases separates
-
I strands
↳ stands coated w/
single-strand binding proteins
strands
rejoining early
-
to
stop
-Primase constructs a
primer ofRNAcomplementary to template,
which gives polymerase something bind to
DNA to
DNA
Polymerase adds nucleotides
direction
to exposed bases
in a
semi
↳ Easily done in one go for leading stand
↳
lagging strand is the other way so is
in small sections which then
formed are
joined
DNA
polymerase also PROOF-READS for errors
↳ Forms backbone on own
↳ Fragments oflagging side joined together by
DNA
ligase
