Unit 6 Investigative Project:
Investigating the Effect of Starch Concentration on Immobilised Amylase Enzyme
Name: ***
Project Supervisor: ***
, Emily Bullas - 246413 Unit 6 Assignment CD
Introduction:
This experiment investigated the effect of starch solution concentration on the effect of immobilised
amylase. The amylase was immobilised using an alginate mixture to set them into beads, which were
submerged in starch solutions of varying concentrations, in a gas syringe. The syringe was lined with
glass wool to prevent the beads from blocking the tubing, which was sealed using a gate clip to keep
the solution inside the syringe until released after 5-minute intervals. Figure 1 below show the
apparatus used for this investigation.
Figure 1: Apparatus for Investigation
Results:
This experiment investigated the effects of starch concentration on the effectiveness of immobilised
amylase, determined through colorimetry. The transmission values are plotted on the line graph in
figure 2 to show the change in starch concentration after each 5-minute exposure with the
immobilised amylase, represented by points 1-5 on the x-axis. The blue, yellow and green colouring
represents the 5%, 4% and 3% starch solutions respectively, with the solid lines showing the actual
results for each repeat and the dashed lines being trendlines for the corresponding results. A
transmission value of 0% would mean that no light is able to pass through the sample, whereas a value
of 100% would mean all of the light is able to pass through the sample. When a line rises over time, it
shows that more light is able to pass through the sample and so the starch concentration is lower.
Investigating the Effect of Starch Concentration on Immobilised Amylase Enzyme
Name: ***
Project Supervisor: ***
, Emily Bullas - 246413 Unit 6 Assignment CD
Introduction:
This experiment investigated the effect of starch solution concentration on the effect of immobilised
amylase. The amylase was immobilised using an alginate mixture to set them into beads, which were
submerged in starch solutions of varying concentrations, in a gas syringe. The syringe was lined with
glass wool to prevent the beads from blocking the tubing, which was sealed using a gate clip to keep
the solution inside the syringe until released after 5-minute intervals. Figure 1 below show the
apparatus used for this investigation.
Figure 1: Apparatus for Investigation
Results:
This experiment investigated the effects of starch concentration on the effectiveness of immobilised
amylase, determined through colorimetry. The transmission values are plotted on the line graph in
figure 2 to show the change in starch concentration after each 5-minute exposure with the
immobilised amylase, represented by points 1-5 on the x-axis. The blue, yellow and green colouring
represents the 5%, 4% and 3% starch solutions respectively, with the solid lines showing the actual
results for each repeat and the dashed lines being trendlines for the corresponding results. A
transmission value of 0% would mean that no light is able to pass through the sample, whereas a value
of 100% would mean all of the light is able to pass through the sample. When a line rises over time, it
shows that more light is able to pass through the sample and so the starch concentration is lower.
