Biology 1404 Exam Chapter 15-17,21 Review Questions and Answers 2023

what is a basic method of extration of DNA? - -1. cells are lysed using a detergent that disrupts plasma membrane 2. cells are treated with protease to destroy protein and RNAase to destroy RNA 3. cells are put in a centrifuge and the supernantant liquid is transferred to a clean tube 4. the DNA is precipitated with ethanol, it forms viscous strands -what is restriction endonucleases? what are they key to?what are Palindromes? - -a defense system for bacteria, it recognizes and cleave a specific sequence, they produce 5, 3 or blunt ends;;;;;;to mapping DNA and producing recombinant DNA;;;;;;racecar, pullup, sees, Was it a cat i saw, dammit, im mad -LOOK AT SLIDES TO SEE PROCESS OF GEL ELECTROPHORESIS - - -what is the Polymerase chain reaction (PCR) used to amplify? what are primers in the PCR for? - -a specific sequence of DNA;;;;; short pieces of DNA complementary to each end of the target sequence which are combined with genomic DNA, taq polymerase, and deoxynucleotides. -what is the Taq polymerase in PCR used for? In general, what ahppends in PCR? LOOK AT NUMBER OF COPIES OF DNA - -it is able to withstand the high temperature used in PCR;;;;; a series of cycles of denaturing DNA, hybridizing primers and extending to make new copies -what is sourthern blotting used to find? LOOK AT PICTURES AND STEPS TO DO IT - -a particular sequnce in a sample of DNA. it allows us to detect individual bands from a large collection -LOOK AT SLEPS ON MOLECULAR CLONING - - -what is Recombinant DNA? LOOK AT