A2: Recombinant DNA Technology Exam Questions with correct answers

Why is it important to use the same restriction endonuclease on DNA samples in an experiment? CORRECT ANSWER Access same base sequence So get fragments with required gene **Suggest why scientists insert a plasmid with EGFP (a gene that codes for bright protein) CORRECT ANSWER Those cells that do not glow brightly have not taken up the plasmid Name the enzyme used to cut a gene from human DNA CORRECT ANSWER Restriction endonuclease Suggest reasons why very few live births result from the many embryos that are implanted. CORRECT ANSWER -Foreign embryo is attacked by antibodies -DNA may be damages -DNA may interfere with proteins It is important that scientists report the results from failed attempts to produce transgenic animals. Why? CORRECT ANSWER -Scientists don't repeat mistakes -Saves time and money When manufacturing large quantities of insulin, why is it better to start by isolating mRNA from pancreas cells rather than DNA from pancreas cells? CORRECT ANSWER -mRNA doesn't have introns -Amount of mRNA>amount of DNA -Specific mRNA found in pancreas cells Name the bond which holds together the 2 strands in a double-stranded DNA molecule. CORRECT ANSWER Hydrogen Bonds