MLS ASCP Microbiology QUESTIONS WITH COMPLETE SOLUTIONS

1 : 10 ratio Correct Answer: The proper blood-to-broth ratio for blood cultures to reduce the antibacterial effects of serum Beta-lactamase Correct Answer: Produced by a penicillin-resistant N. gonorrhoeae Columbia CNA with 5% sheep blood Correct Answer: Selective medium used for the isolation of gram-positive microorgnisms Indole test Correct Answer: used for determining an organism's ability to produce indole from deamination of tryptophan JEMBEC system Correct Answer: is a transport and inoculation medium used for direct plating of specimens for N gonorrhoeae. Chocolate based selective medium is inoculated with the specimen. This is placed in an impermeable plastic bag with a bicarbonate-sodium citrate pellet to generate a carbon dioxide rich environment. Martin Lewis agar Correct Answer: Chocolate agar base containing vancomycin, colistin, anisomycin, and trimethoprim for better inhibition of gram-positive organisms and candida. Differential medium Correct Answer: aids in the presumptive identification of organisms based on their apperance SPS Correct Answer: used as anticoagulant in blood cultures to inactivate neutrophils and components of serum complement Blood culture Correct Answer: Typhoid fever can be confirmed by this culture Regan-Lowe agar Correct Answer: best medium for culture for Bordetella pertussis Francisella tularensis Correct Answer: most rapid method for detection is PCR synovial infections Correct Answer: Anaerobic susceptibility testing is helpful Schlichter test Correct Answer: is used to monitor bactericidal activity during antimicrobic therapy in cases of endocaditis Moraxella osloensis Correct Answer: is a gram-negative coccobacillus that is often plump and occurs in pairs and demonstrates a morphology similar to Neisseria Extra-intestinal isolates of Salmonella Correct Answer: should be tested for resistance to nalidixic acid and if resistant report the ciprofloxacin as resistant