UNIT 6B _ PROJECT
PLAN
Plan, method and Health & Safety P3,
M3, D2
, Unit 6B __ Planning and health & safety
Contents
Methods.....................................................................................................................1
Equipment List..........................................................................................................12
Contingency plans......................................................................................................13
Risk assessment........................................................................................................15
References................................................................................................................17
Methods
Preparing Solid Media (agar plates) Using Aseptic Technique
Method
1| Page
, Unit 6B __ Planning and health & safety
1. Wash your hands thoroughly with soap and water (not just sanitiser). Do not touch your face or hair
after this point or you will need to do so again.
2. Wipe down the desk with antibacterial spray and tissue roll.
3. Turn the Bunsen burner on, working underneath it at all times during the practical.
4. Carefully weigh out 3g in of nutrient agar powder into the small beaker and add 100cm 3 to the
measuring cylinder. Add some of the water to the agar powder in the beaker and stir it until all
dissolved and then add the liquid to the bottle and the rest of the distilled water.
5. Stir with a glass rod to mix then secure the cap on the bottle.
6. Place the bottle in the autoclave where it will be heated under pressure for the next day so that it
reaches a high enough temperature to kill any microorganisms and sterilise the agar.
7. Allow the agar to cool to around 70oC (you should be able to put you hand round it at this temperature)
then carefully pour it into four sterile petri dishes as demonstrated by your teacher using aseptic
technique
8. Repeat step 8 until you have made 12 petri dishes in total.
9. Leave the agar plates to cool. Do not move them until the agar has fully set.
10. Seal in two places with Sellotape, write your initials and the date around the rim in permanent marker.
11.
Preparing a Bacterial Culture
12. Bacteria to be cultivated are inoculated using a loop or swab into a culture medium (a liquid full of
nutrients) and grown overnight at 25 degrees Celsius.
13. Transfer one colon from the agar plate into a bottle of culture for growth overnight using a flamed loop
and aseptic technique.
Making dilution for ginger
1. Using 4 bottles of 20ml (McCartney) label using marker pen on each bottle for distilled water, 1%,
10%, and 100%
2. Using syringes (0.1-1ml and 0.5-5.0ml) to get the liquid out of the ginger bottle and then add the
correct ratio of the ginger liquid and water to each of the bottles as it done in the table.
3. Then using hole puncher and paper make 6 paper discs for each bottle and 12 paper discs for the
distilled water and put them inside the bottles.
Concentration (%) Volume of ginger liquid (ml) Volume of water (ml)
Distilled water 0 10
1 0.1 9.9
10 1 9
100 10 0
Making dilution for hand gel
1. Using 3 bottles of 20ml (McCartney) label using marker pen on each bottle for distilled water, 1%,
10%, and 100%
2| Page
PLAN
Plan, method and Health & Safety P3,
M3, D2
, Unit 6B __ Planning and health & safety
Contents
Methods.....................................................................................................................1
Equipment List..........................................................................................................12
Contingency plans......................................................................................................13
Risk assessment........................................................................................................15
References................................................................................................................17
Methods
Preparing Solid Media (agar plates) Using Aseptic Technique
Method
1| Page
, Unit 6B __ Planning and health & safety
1. Wash your hands thoroughly with soap and water (not just sanitiser). Do not touch your face or hair
after this point or you will need to do so again.
2. Wipe down the desk with antibacterial spray and tissue roll.
3. Turn the Bunsen burner on, working underneath it at all times during the practical.
4. Carefully weigh out 3g in of nutrient agar powder into the small beaker and add 100cm 3 to the
measuring cylinder. Add some of the water to the agar powder in the beaker and stir it until all
dissolved and then add the liquid to the bottle and the rest of the distilled water.
5. Stir with a glass rod to mix then secure the cap on the bottle.
6. Place the bottle in the autoclave where it will be heated under pressure for the next day so that it
reaches a high enough temperature to kill any microorganisms and sterilise the agar.
7. Allow the agar to cool to around 70oC (you should be able to put you hand round it at this temperature)
then carefully pour it into four sterile petri dishes as demonstrated by your teacher using aseptic
technique
8. Repeat step 8 until you have made 12 petri dishes in total.
9. Leave the agar plates to cool. Do not move them until the agar has fully set.
10. Seal in two places with Sellotape, write your initials and the date around the rim in permanent marker.
11.
Preparing a Bacterial Culture
12. Bacteria to be cultivated are inoculated using a loop or swab into a culture medium (a liquid full of
nutrients) and grown overnight at 25 degrees Celsius.
13. Transfer one colon from the agar plate into a bottle of culture for growth overnight using a flamed loop
and aseptic technique.
Making dilution for ginger
1. Using 4 bottles of 20ml (McCartney) label using marker pen on each bottle for distilled water, 1%,
10%, and 100%
2. Using syringes (0.1-1ml and 0.5-5.0ml) to get the liquid out of the ginger bottle and then add the
correct ratio of the ginger liquid and water to each of the bottles as it done in the table.
3. Then using hole puncher and paper make 6 paper discs for each bottle and 12 paper discs for the
distilled water and put them inside the bottles.
Concentration (%) Volume of ginger liquid (ml) Volume of water (ml)
Distilled water 0 10
1 0.1 9.9
10 1 9
100 10 0
Making dilution for hand gel
1. Using 3 bottles of 20ml (McCartney) label using marker pen on each bottle for distilled water, 1%,
10%, and 100%
2| Page
