Unit 2: Practical Scientific Procedure and Techniques. Learning aim C: Undertake
chromatographic techniques to identify components in mixtures.
Index
Introduction,
Chromatography,
Risk Assessment,
Methods,
Results & Rf Valuations,
Conclusion,
Evaluation,
Introduction
This report will show how to correctly use chromatographic techniques to produce chromatograms,
and explain the use of chromatographic techniques to separate mixtures. An analysis of the results
received in the experiment that was done and how they relate to the factors that affect the separation
of mixtures to the quality of results obtained, and finally evaluate the chromatographic techniques
used in relation to outcomes and suggest improvements.
Chromatography
Chromatography is a technique used to separate molecules based on how they tend to cling to or
dissolve in various solids, liquids and gases. It is done in two phases, the mobile phase and stationary
phase where substances will move at different rates in the mobile phase depending on their partition.
Chromatography is based on the principle where molecules in a mixture applied onto the surface or
into the solid, and fluid stationary phase is separating from each other while moving with the aid of a
mobile phase.
Paper Chromatography is able to separate substances as the solvent that the chromatography paper
is placed in will soak up the paper and carry the substances with it and because different components
of the mixture will move at different rates this therefore separates the substances out.
There are different techniques of chromatography including Paper, Thin layer and High performance
liquid chromatography. Paper chromatography is an inexpensive method that involves using a solvent
a piece of chromatography paper and a specific sample. The solvent will carry the sample up the
paper and reveal how many substances that one sample contains. TLC chromatography is a
technique performed on a piece of glass or plastic coated in silica gel usually referred to as a TLC
plate. It is a more accurate version of the paper chromatography.
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chromatographic techniques to identify components in mixtures.
Index
Introduction,
Chromatography,
Risk Assessment,
Methods,
Results & Rf Valuations,
Conclusion,
Evaluation,
Introduction
This report will show how to correctly use chromatographic techniques to produce chromatograms,
and explain the use of chromatographic techniques to separate mixtures. An analysis of the results
received in the experiment that was done and how they relate to the factors that affect the separation
of mixtures to the quality of results obtained, and finally evaluate the chromatographic techniques
used in relation to outcomes and suggest improvements.
Chromatography
Chromatography is a technique used to separate molecules based on how they tend to cling to or
dissolve in various solids, liquids and gases. It is done in two phases, the mobile phase and stationary
phase where substances will move at different rates in the mobile phase depending on their partition.
Chromatography is based on the principle where molecules in a mixture applied onto the surface or
into the solid, and fluid stationary phase is separating from each other while moving with the aid of a
mobile phase.
Paper Chromatography is able to separate substances as the solvent that the chromatography paper
is placed in will soak up the paper and carry the substances with it and because different components
of the mixture will move at different rates this therefore separates the substances out.
There are different techniques of chromatography including Paper, Thin layer and High performance
liquid chromatography. Paper chromatography is an inexpensive method that involves using a solvent
a piece of chromatography paper and a specific sample. The solvent will carry the sample up the
paper and reveal how many substances that one sample contains. TLC chromatography is a
technique performed on a piece of glass or plastic coated in silica gel usually referred to as a TLC
plate. It is a more accurate version of the paper chromatography.
1
