BTEC 6104 Exam QUESTIONS & ANSWERS 2022 UPDATE

BTEC 6104 Exam Terms in this set (75) The use of living cells or the molecular components of their metabolism to produce desired products is called ______________________? A. Cell manufacturing B. Bioprocessing C. Molecular processing D. None of the above Bioprocessing Upstream processing includes the purification and polishing of your protein product. A. True B. False False What makes the discovery phase of a new bioproduct expensive? The time involved in research and development Two examples of bioprocessing products Cheese, Antibiotics This study source was downloaded by from CourseH on :33:33 GMT -06:00 Sterilization and containment are critical in industrial scale bioprocessing operations. A. True B. False True Name 3 of the common biological materials used in Bioprocessing Bacteria, Viruses, Yeast Upstream processing Recombinant DNATechnology and Cell Growth Downstream Processing Target Purification and Polishing Genetic manipulation is the first step in bioprocessing. A. True B. False True What is the name of the gene you will be working with in class? opd Quality Assurance covers documentation of things like Product yield, safety What size bioreactor does BTEC 4101 use? 3 L This study source was downloaded by from CourseH on :33:33 GMT -06:00 How large can a pilot scale bioreactor be? 10,000 L Biotherapeutics Therapeutic materials produced via biological means/methods Example of transgenic plant Pest resistant crops, virus resistant tobacco Examples of Biotherapeutic Vaccines, insulin Examples of Bioenergy Biodiesel, bioethanol Examples of Microbial culture Industrial enzymes, organic acids The majority of therapeutic proteins are produced using ______ cells CHO Gene therapy is most often carried out using a ______ vector Viral Two ways that biopharmaceuticals differ from standard pharmaceuticals Development time, regulation Three examples of transgenic animals and their bioproducts Goats - Spider silk Rats - cancer cell cultures Pigs - human insulin This study source was downloaded by from CourseH on :33:33 GMT -06:00 When biomass is burned at high temperatures using controlled amounts of gas/steam it produces synthetic fuel called ______ Syngas A medical therapy replacing an enzyme in patients is called ______ Enzyme replacement therapy What does CHO stand for? Chinese hamster ovary Texas produces the most of what bioengineered crop? Cotton The bioproduct ______ has been produced for hundreds of years Beer/wine Assign BSL - E. coli BSL 1 Assign BSL - Encephalitis BSL - 3 Assign BSL - Salmonellae BSL 2 Assign BSL - Ebola BSL 4 The state of being free from disease-causing contaminants such as bacteria, viruses, fungi, and parasites Asepsis This study source was downloaded by from CourseH on :33:33 GMT -06:00 Match to PPE - Absorption Sleeves Match to PPE - Ingestion Serological pipettor Match to PPE - Inhalation BSC Class II Match to PPE - Injection Glass Disposal For sterility assurance a sample/material must have a 1:100,000 probability of surviving microorganisms. A. True B. False False Give example of sterilization method: Heat Flame Give example of sterilization method: Radiation UV light, Gamma Give example of sterilization method: Chemical Ethanol, isopropanol, bleach Give example of sterilization method: Steam Autoclave Bacteria are _____ than viruses, and fungi are _____ than bacteria larger, larger This study source was downloaded by from CourseH on :33:33 GMT -06:00 In positive pressure systems the air outside the room is at a higher pressure than the air inside the room. A. True B. False False What is the difference between a disinfectant and a decontaminant? Disinfectants are not 100% sterile and cannot kill spores The number one source of environmental contamination is ______ and the second most likely source is ______ Personnel, equipment Ways that bioprocessing facilities can control for contamination are: Filtered air, clean rooms, and layout What does HEPA stand for? High Efficiency Particulate Air How many BSL 4 facilities are in TExas? 2 Name a chemical used in a CIP cocktail SDS, Acid, Base, alcohol The cleanest ISO clean air designation/level is Level 5/ Level 100 This study source was downloaded by from CourseH on :33:33 GMT -06:00 What university had a student researcher twice treated for Lyme disease? Texas A&M In Growth Associated Product formation, product expression is linked to energy metabolism. A. True B. False True Batch Bioreactor Operation Mode features All nutrients are added before inoculation, toxic waste products influence growth rate, easy to maintain sterility Continuous Bioreactor Operational Mode features Flow rate is equal to dilution rate, no fluctuations in nutrients, metabolites or cell numbers in vessel Shear damage damage to cell membrane by applied mechanical forces Why is Optical Density not a reliable means of estimating cell viability? Dead cells contribute to the overall count Example of Media Component: Carbohydrate Glucose Example of Media Component: Fatty acids/lipids Cholesterol This study source was downloaded by from CourseH on :33:33 GMT -06:00 Example of Media Component: Inorganic salt Potassium Example of Media Component: Vitamins B6/12, ascorbic acid Two advantages of using disposables in the laboratory Maintain sterility, Less time consuming Examples of Anchorage culture types Roller bottle, cell factory plate Examples of Suspension culture types Biofermentors, flask Example of Anchorage and Suspension culture type Microcarriers Difference between defined and undefined media Defined media has all content and quantities known Cell density is measured at an OD of approximately ____ nm 600 In a balanced metabolic system, Oxygen Transfer Rate = Oxygen Uptake Rate For PID Control, if SP is _________ PV, then Output is increased greater than List 4 factors that directly influence growth in a biofermentor temperature, pH, dissolved oxygen, carbon source This study source was downloaded by from CourseH on :33:33 GMT -06:00 Some of the ways to obtain the desired D.O. value are Larger vessel surface area, Higher agitation rates, Aerate the vessel What are two disadvantages of growing culture in a flask versus a bioreactor? Added labor and time, Cost What are the benefits of computerized data logging over manual records? Less mistakes, more accurate data _______ sensors must be capable of detection over extended periods of time (amount of time necessary for the completion of the bioprocess). These sensors may also be autoclavable. in situ The culture inside a bioreactor chamber can be cooled using a cooling coil, jacketed water chamber A _____ adds gases to the biofermentor at varying volumes and times. TMFC This study source was downloaded by from CourseH on :33:33 GMT -06:00 Powered by TCPDF ()