BioChem 210 Exam Module 8
Questions and Ansẉers
2026\2027 update
1. The process of synthesizing RNA from a DNA template is called:
A) Replication
B) Transcription
C) Translation
D) Reverse transcription
Answer: B
2. In transcription, the DNA strand that serves as the template for
RNA synthesis is called the:
A) Coding strand
B) Sense strand
C) Template strand (antisense strand)
D) Lagging strand
Answer: C
3. The enzyme responsible for synthesizing RNA in prokaryotes is:
A) DNA polymerase III
B) RNA polymerase
C) Primase
,D) Reverse transcriptase
Answer: B
4. During transcription, RNA is synthesized in which direction?
A) 3' to 5'
B) 5' to 3'
C) N-terminus to C-terminus
D) Bidirectionally
Answer: B
5. In prokaryotes, which sigma (σ) factor is primarily responsible for
recognizing standard housekeeping gene promoters?
A) Sigma 70 (σ70)
B) Sigma 32 (σ32)
C) Sigma 54 (σ54)
D) Alpha factor
Answer: A
6. The prokaryotic promoter region contains two highly conserved
sequences. The -10 region is known as the:
A) Shine-Dalgarno sequence
B) Pribnow box (TATAAT)
C) -35 box (TTGACA)
D) Kozak sequence
Answer: B
, 7. Unlike DNA polymerase, RNA polymerase does not possess:
A) Helicase activity
B) 3' to 5' exonuclease (proofreading) activity
C) 5' to 3' polymerase activity
D) The ability to unwind DNA
Answer: B
8. In prokaryotes, transcription and translation are said to be
"coupled." This means:
A) They occur in different cellular compartments.
B) Translation begins before transcription is complete.
C) They use the same enzyme.
D) Transcription requires translating ribosomes to proceed.
Answer: B
9. Which antibiotic specifically inhibits prokaryotic RNA polymerase
by binding to the beta subunit?
A) Streptomycin
B) Tetracycline
C) Rifampicin (Rifampin)
D) Penicillin
Answer: C
10. In rho (ρ)-independent termination of transcription in E. coli,
termination relies on the formation of a:
Questions and Ansẉers
2026\2027 update
1. The process of synthesizing RNA from a DNA template is called:
A) Replication
B) Transcription
C) Translation
D) Reverse transcription
Answer: B
2. In transcription, the DNA strand that serves as the template for
RNA synthesis is called the:
A) Coding strand
B) Sense strand
C) Template strand (antisense strand)
D) Lagging strand
Answer: C
3. The enzyme responsible for synthesizing RNA in prokaryotes is:
A) DNA polymerase III
B) RNA polymerase
C) Primase
,D) Reverse transcriptase
Answer: B
4. During transcription, RNA is synthesized in which direction?
A) 3' to 5'
B) 5' to 3'
C) N-terminus to C-terminus
D) Bidirectionally
Answer: B
5. In prokaryotes, which sigma (σ) factor is primarily responsible for
recognizing standard housekeeping gene promoters?
A) Sigma 70 (σ70)
B) Sigma 32 (σ32)
C) Sigma 54 (σ54)
D) Alpha factor
Answer: A
6. The prokaryotic promoter region contains two highly conserved
sequences. The -10 region is known as the:
A) Shine-Dalgarno sequence
B) Pribnow box (TATAAT)
C) -35 box (TTGACA)
D) Kozak sequence
Answer: B
, 7. Unlike DNA polymerase, RNA polymerase does not possess:
A) Helicase activity
B) 3' to 5' exonuclease (proofreading) activity
C) 5' to 3' polymerase activity
D) The ability to unwind DNA
Answer: B
8. In prokaryotes, transcription and translation are said to be
"coupled." This means:
A) They occur in different cellular compartments.
B) Translation begins before transcription is complete.
C) They use the same enzyme.
D) Transcription requires translating ribosomes to proceed.
Answer: B
9. Which antibiotic specifically inhibits prokaryotic RNA polymerase
by binding to the beta subunit?
A) Streptomycin
B) Tetracycline
C) Rifampicin (Rifampin)
D) Penicillin
Answer: C
10. In rho (ρ)-independent termination of transcription in E. coli,
termination relies on the formation of a:
