Lab 7- Photosynthesis pigments,
chromatography, and spectrophotometry
2026/2027 With Complete Solutiions
-Paper chromatography is a useful technique for separating and identifying pigments and other
molecules from cell extracts that contain a complex mixture of molecules
-The solvent moves up the paper by capillary action, which occurs due to attraction between solvent
molecules and the paper and the solvent molecules and each other. When the solvent moves up the
paper, it carries the solute. Pigments are carried at different rates because they have varying solubilities
and because they have different attraction levels to the paper through H-bonds - correct
answer<✔✔💖💖What is paper chromatography used for and how does it work?
Beta-carotene, xanthophylls, chlorophyll a, chlorophyll b, anthocyanins - correct
answer<✔✔💖💖What are the five pigments discussed in this lab?
-The most abundant carotene in plants, carried along the solvent front because it is very soluble and
forms no H-bonds with cellulose - correct answer<✔✔💖💖Describe Beta-carotene
-Contain oxygen, found further from the solvent front than beta-carotene because they are less soluble
and have some H-bonding with the cellulose - correct answer<✔✔💖💖Describe xanthophylls
-Chlorophylls contain oxygen and nitrogen and are more tightly bound to the paper, however it is
soluble
-Chlorophyll a is bluish-green, primary photosynthetic pigment in plants; two molecules of chlorophyll a
are located at the reaction center of photosystems
-Chlorophyll b is yellowish-green - correct answer<✔✔💖💖Describe chlorophyll (a and b)
-Water-soluble pigment, reddish in color, often trails behind or is left at the starting line - correct
answer<✔✔💖💖Describe anthocyanins
chromatography, and spectrophotometry
2026/2027 With Complete Solutiions
-Paper chromatography is a useful technique for separating and identifying pigments and other
molecules from cell extracts that contain a complex mixture of molecules
-The solvent moves up the paper by capillary action, which occurs due to attraction between solvent
molecules and the paper and the solvent molecules and each other. When the solvent moves up the
paper, it carries the solute. Pigments are carried at different rates because they have varying solubilities
and because they have different attraction levels to the paper through H-bonds - correct
answer<✔✔💖💖What is paper chromatography used for and how does it work?
Beta-carotene, xanthophylls, chlorophyll a, chlorophyll b, anthocyanins - correct
answer<✔✔💖💖What are the five pigments discussed in this lab?
-The most abundant carotene in plants, carried along the solvent front because it is very soluble and
forms no H-bonds with cellulose - correct answer<✔✔💖💖Describe Beta-carotene
-Contain oxygen, found further from the solvent front than beta-carotene because they are less soluble
and have some H-bonding with the cellulose - correct answer<✔✔💖💖Describe xanthophylls
-Chlorophylls contain oxygen and nitrogen and are more tightly bound to the paper, however it is
soluble
-Chlorophyll a is bluish-green, primary photosynthetic pigment in plants; two molecules of chlorophyll a
are located at the reaction center of photosystems
-Chlorophyll b is yellowish-green - correct answer<✔✔💖💖Describe chlorophyll (a and b)
-Water-soluble pigment, reddish in color, often trails behind or is left at the starting line - correct
answer<✔✔💖💖Describe anthocyanins
