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Summary notes for CIE IGCSE Biology Topic 12: Respiration

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Complete revision notes for Topic 12 of the CIE IGCSE Biology course: Respiration. Explanations with diagrams for every specification point. These notes are written for candidates taking the Extended paper.

Institution
GCSE
Module
Biology









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April 9, 2021
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Respiration

12.1 Respiration

Respiration is a chemical process that involves the breakdown of nutrient molecules
(specifically glucose) in order to release the energy stored within the bonds of these
molecules.

Respiration can take place with oxygen (aerobically) or without oxygen (anaerobically).
Much less energy is released for each glucose molecule broken down anaerobically
compared to the energy released when it is broken down aerobically.

Respiration occurs in all living cells. Most of the chemical reactions in aerobic respiration
take place in the mitochondria.

State the uses of energy in the body of humans: muscle contraction, protein synthesis, cell
division, active transport, growth, the passage of nerve impulses and the maintenance of a
constant body temperature

State that respiration involves the action of enzymes in cells

Respiration is a series of reactions that are controlled by enzymes. You need to be able to
state this in an exam!

12.2 Aerobic respiration

Define aerobic respiration as the chemical reactions in cells that use oxygen to break down
nutrient molecules to release energy

Aerobic respiration requires oxygen and is defined as the chemical reactions in cells that use
oxygen to break down nutrient molecules to release energy.

It is the complete breakdown of glucose to release a relatively large amount of energy for
use in cell processes.

It produces carbon dioxide and water as well as releasing useful cellular energy.

State the word equation for aerobic respiration as glucose + oxygen → carbon dioxide +
water

State the balanced chemical equation for aerobic respiration as
C6H12O6 + 6O2 → 6CO2 + 6H2O

There are usually 3 marks given for the aerobic respiration chemical equation in an exam:
 One for getting the correct formula for glucose and oxygen
 One for getting the correct formula for carbon dioxide and water

,  One for balancing the equation correctly

Investigate the effect of temperature on the rate of respiration of germinating seeds

To investigate the effect of temperature on the rate of respiration of germinating seeds the
respirometer can be set up and the tubes submerged in a series of water baths set at
different temperatures, e.g. 10℃, 15℃, 20℃, 25℃, 30°C
 The seeds should be kept in the water bath for 15 minutes before the start of the
experiment to ensure they have acclimated to the temperature
 As respiration is an enzyme-controlled reaction, it is unlikely to work faster beyond
around 40℃ as the enzymes will denature

Investigate the uptake of oxygen by respiring organisms, such as arthropods and
germinating seeds

We can investigate aerobic respiration in living organisms by measuring the amount of
oxygen that they take from the air
 This is done by measuring the change in volume in an enclosed tube containing the
organisms

However, as they respire the organisms release carbon dioxide, which increases the gas
volume
 The carbon dioxide must therefore be removed from the tube using a chemical
like soda lime or sodium hydroxide, otherwise it will make the experiment results
inaccurate

Any small organisms can be used in the apparatus, including seeds or arthropods.

The apparatus (shown below) is known as a respirometer:

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