Portage Learning Bio 171 - Microbiology NOTEBOOK .pdf 1 Portage Learning Bio 171 - Microbiology NOTEBOOK .pdf
Portage Learning
Bio 171 - Microbiology
Cassidy Tate
Title: CT#1 Introduction to Medical Microbiology - Keeping a lab notebook
Objective:
Cultivation of lab samples, Identification of samples,and Evaluation of samples.
Determining what kind of bacteria or pathogen a pt. may have
Establishing an organized template for keeping experimental notes.
Procedure: Clean and provide a sterile environment
This is where each step of protocol is recorded
Clearly indicate deviations from standard protocol that occur during experiment write this in red.
Notes:
Equipment used: For cleaning- Autoclave 125 degrees celsius
For growing samples- fixed incubator 37 degrees celsius,shaker incubator 37 degrees celsius
For visualizing - microscopy
Storing- refrigerator 4 degrees celsius which slows sample growth and prolongs analyzation og
the samples.
Rules in Lab: no food or drinks due to infection control, Personal Protective equipment is
important - gloves,lab coat, goggles or glasses. Never leave a lab with PPE on it must stay in the
lab.
Results:
Summary of final experimental outcome. Sterile condition is achieved with an autoclave in a
in a matter of minutes with hot air it would take several hours.
Title: CT02 Basics of Microscopy
Objective: Familiarize yourself with the basic components of a light microscope as well as how
to load a sample for viewing.
Procedure:
1. Review parts and components- Eyepiece,neck,objectives,revolving nosepiece,
stage,stagecontrols,stage clip, fine focus, iris diaphragm, light source, base
2. Load sample slide onto microscope- pinch metal bars of clamp holder to open the arms of
stage place the glass slide and slowly release metal bars and it will hold the slide in place.
This study source was downloaded by 1827175 from cliffsnotes.com on 01-08-2026 18:41:37 GMT -06:00
Portage Learning Bio 171 - Microbiology NOTEBOOK Portage
.pdfLearning Bio 171 - Microbiology NOTEBOOK
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.pdfLearning Bio 171 - Microbiology NOTEBOOK .pdf
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, Portage Learning Bio 171 - Microbiology NOTEBOOK .pdf 2 Portage Learning Bio 171 - Microbiology NOTEBOOK .pdf
3. Select magnification - 4 different objectives to change magnification spin each one to
choose correct magnification. 4x,10x,40x,100x are all of the magnification options
4. Make necessary adjustments to optimize sample visualization- grab and pull eye pieces to
fit the comfort of your eyes. Fine focus may help zooming in as well. Iris or diaphragm
will control how much light is let into the eyepiece of scope.
If a cell is 15 mm in diameter use 40x on objective,and 5x eyepiece the cell will appear to the
eye 200 times larger. Start on the lowest power possible with magnification if unsure of which to
use. Make sure to have a single circle when looking through the ocular pieces. The pointer is
used to point at a specific part of the sample move the pointer by moving sample
Notes: grab microscope by the neck and hand under the base when carrying it
Keep microscope on flat surface and steady
Results: magnification = total power of objective x the eye piece
Title:
CT 03 Mounting Techniques
Objective: Microscopic examination of bacterial samples through various staining techniques.
Identify color and shape of given samples
Procedure:
Wear proper PPE
Dry mount
1. Clean glass slide (70% ethanol)
2. Circle area on slide for easy location of specimen
3. Apply organism to slide - if from a culture use a sterile loop to spread onto slide, if from
plate use sterile loop to pick colonies and mix with a drop of distilled water
4. Air dry at room temp. Until all moisture has evaporated.
5. Flood slide with crystal violet for 30-60 seconds
6. Rinse with water
7. Decolorize with alcohol
8. Rinse with water
9. Decolorize with alcohol
10. Rinse with water
11. Counterstain with safranin for 30 seconds (red/pink dye)
12. Rinse with water
13. Blot dry and examine under microscope
14. Heat fix all samples
15. Place slide in tray with dye
This study source was downloaded by 1827175 from cliffsnotes.com on 01-08-2026 18:41:37 GMT -06:00
Portage Learning Bio 171 - Microbiology NOTEBOOK Portage
.pdfLearning Bio 171 - Microbiology NOTEBOOK
Portage
.pdfLearning Bio 171 - Microbiology NOTEBOOK .pdf
https://www.cliffsnotes.com//study-notes/5883192
Portage Learning
Bio 171 - Microbiology
Cassidy Tate
Title: CT#1 Introduction to Medical Microbiology - Keeping a lab notebook
Objective:
Cultivation of lab samples, Identification of samples,and Evaluation of samples.
Determining what kind of bacteria or pathogen a pt. may have
Establishing an organized template for keeping experimental notes.
Procedure: Clean and provide a sterile environment
This is where each step of protocol is recorded
Clearly indicate deviations from standard protocol that occur during experiment write this in red.
Notes:
Equipment used: For cleaning- Autoclave 125 degrees celsius
For growing samples- fixed incubator 37 degrees celsius,shaker incubator 37 degrees celsius
For visualizing - microscopy
Storing- refrigerator 4 degrees celsius which slows sample growth and prolongs analyzation og
the samples.
Rules in Lab: no food or drinks due to infection control, Personal Protective equipment is
important - gloves,lab coat, goggles or glasses. Never leave a lab with PPE on it must stay in the
lab.
Results:
Summary of final experimental outcome. Sterile condition is achieved with an autoclave in a
in a matter of minutes with hot air it would take several hours.
Title: CT02 Basics of Microscopy
Objective: Familiarize yourself with the basic components of a light microscope as well as how
to load a sample for viewing.
Procedure:
1. Review parts and components- Eyepiece,neck,objectives,revolving nosepiece,
stage,stagecontrols,stage clip, fine focus, iris diaphragm, light source, base
2. Load sample slide onto microscope- pinch metal bars of clamp holder to open the arms of
stage place the glass slide and slowly release metal bars and it will hold the slide in place.
This study source was downloaded by 1827175 from cliffsnotes.com on 01-08-2026 18:41:37 GMT -06:00
Portage Learning Bio 171 - Microbiology NOTEBOOK Portage
.pdfLearning Bio 171 - Microbiology NOTEBOOK
Portage
.pdfLearning Bio 171 - Microbiology NOTEBOOK .pdf
https://www.cliffsnotes.com//study-notes/5883192
, Portage Learning Bio 171 - Microbiology NOTEBOOK .pdf 2 Portage Learning Bio 171 - Microbiology NOTEBOOK .pdf
3. Select magnification - 4 different objectives to change magnification spin each one to
choose correct magnification. 4x,10x,40x,100x are all of the magnification options
4. Make necessary adjustments to optimize sample visualization- grab and pull eye pieces to
fit the comfort of your eyes. Fine focus may help zooming in as well. Iris or diaphragm
will control how much light is let into the eyepiece of scope.
If a cell is 15 mm in diameter use 40x on objective,and 5x eyepiece the cell will appear to the
eye 200 times larger. Start on the lowest power possible with magnification if unsure of which to
use. Make sure to have a single circle when looking through the ocular pieces. The pointer is
used to point at a specific part of the sample move the pointer by moving sample
Notes: grab microscope by the neck and hand under the base when carrying it
Keep microscope on flat surface and steady
Results: magnification = total power of objective x the eye piece
Title:
CT 03 Mounting Techniques
Objective: Microscopic examination of bacterial samples through various staining techniques.
Identify color and shape of given samples
Procedure:
Wear proper PPE
Dry mount
1. Clean glass slide (70% ethanol)
2. Circle area on slide for easy location of specimen
3. Apply organism to slide - if from a culture use a sterile loop to spread onto slide, if from
plate use sterile loop to pick colonies and mix with a drop of distilled water
4. Air dry at room temp. Until all moisture has evaporated.
5. Flood slide with crystal violet for 30-60 seconds
6. Rinse with water
7. Decolorize with alcohol
8. Rinse with water
9. Decolorize with alcohol
10. Rinse with water
11. Counterstain with safranin for 30 seconds (red/pink dye)
12. Rinse with water
13. Blot dry and examine under microscope
14. Heat fix all samples
15. Place slide in tray with dye
This study source was downloaded by 1827175 from cliffsnotes.com on 01-08-2026 18:41:37 GMT -06:00
Portage Learning Bio 171 - Microbiology NOTEBOOK Portage
.pdfLearning Bio 171 - Microbiology NOTEBOOK
Portage
.pdfLearning Bio 171 - Microbiology NOTEBOOK .pdf
https://www.cliffsnotes.com//study-notes/5883192
