QUESTIONS AND ANSWERS RATED
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Whatmismamrestrictionmenzyme?m-
mCORRECTmANSWERSmAnmenzymemthatmcutsmDNAmatmamspecificmsequencemofmnucleotides
Whymaremrestrictionmenzymesmimportant?m-mCORRECTmANSWERSmTheymaremspecific
Whichmrestrictionmenzymesmweremusedminmthismexperiment?m-
mCORRECTmANSWERSmEcoRI,mBamHI,mandmHindIII
Whichmbacteriamdidmeachmofmthemrestrictionmenzymesmcomemfrom?m-
mCORRECTmANSWERSmBammHI:mBacillusmamyloliquefaciensm
EcomRI:mEscherichiamcolimRYm13m
HindmIII:mHaemophilusminflenzaemRd
Whatmpercentagemwasmthemagarosemgelmthatmyoumran?m-mCORRECTmANSWERSm0.8%
WhatmwasmusedmtomstainmthemDNAmbands?m-mCORRECTmANSWERSmEthidiummBromidem(OrmSYBR-
Green)
Whatmwasmthempurposemofmthemcontrol?m-
mCORRECTmANSWERSmTomusemasmamformmofmcomparisonmtomthemothermDNAmmolecules
Whatmaremtwomfunctionsmofmloadingmdye?m-
mCORRECTmANSWERSm1.mThesemdyesmwillmpermitmtheminvestigatormtomfollowmthemprogressmofmthem
separationmduringmanmelectrophoreticmrun