VOLTAGE-GATED ION CHANNELS S MEDIATORS
OF NEURONAL SIGNALLING
What will be looking at in this lecture:
How the NS works at the molecular level?
Show examples of comparative physiology
Learning objective:
1. To illustrate how research on a variety of organisms ranging from prokaryotes.
Squids, fruit-flies, electric eel have enabled an understanding of the molecular
mechanisms by which neurons generate nerve impulses (AP) and trigger secretion of
neurotransmitters
2. Briefly discuss translational relevance of this research in understanding causes of
neurological disorders.
Ion channels
1. Leak channel
- Open all the time
- Vital in RMP
2. Gated-channel
- Ligand
- Voltage
- Mechanical
- Open or close to specific electrochemical neuromechanical signals
- Require change in conformation of chemical structure
3 types of receptors
1. Ion-channel-linked receptor
- Leaky
- Gated
Voltage
Mechanical
Ligand
2. G-protein (also called transmembrane receptor)
3. Enzyme- linked
-
Part 1: introduction to AP and the techniques and tools used to study the
electrophysiology of neurons.
If research scientists only focused on rats and mice as model organisms across
various science disciplines, then little progress would have been made regarding the
understanding of animal physiology.
Hodgkin and Huxley use of the giant squid axon is the most famous example of
Krogh’s principle
, The giant squid axon is a model organism in neurobiology and the starting point to
understanding AP in the NS.
in 1939, Hodgkin and Huxley inserted an intracellular electrode inside an axon and
recoded the very first AP.
In 1963, Nobel prize for physiology was awarded to Eccles, Hodgkin and Huxley for
their discoveries concerning the ionic mechanisms involved in excitation and
inhibition in the peripheral and central portions on the nerve cell membrane.
How to take the recording of the resting membrane potential of a squid giant axon?
Recording microelectrode and reference electrode are outside the axon.
Place recording microelectrode inside the squid axon
Voltmeter will start to measure inside the membrane.
Voltmeter reads inside the membrane as negative and outside as positive
RMP is -65Mv.
OF NEURONAL SIGNALLING
What will be looking at in this lecture:
How the NS works at the molecular level?
Show examples of comparative physiology
Learning objective:
1. To illustrate how research on a variety of organisms ranging from prokaryotes.
Squids, fruit-flies, electric eel have enabled an understanding of the molecular
mechanisms by which neurons generate nerve impulses (AP) and trigger secretion of
neurotransmitters
2. Briefly discuss translational relevance of this research in understanding causes of
neurological disorders.
Ion channels
1. Leak channel
- Open all the time
- Vital in RMP
2. Gated-channel
- Ligand
- Voltage
- Mechanical
- Open or close to specific electrochemical neuromechanical signals
- Require change in conformation of chemical structure
3 types of receptors
1. Ion-channel-linked receptor
- Leaky
- Gated
Voltage
Mechanical
Ligand
2. G-protein (also called transmembrane receptor)
3. Enzyme- linked
-
Part 1: introduction to AP and the techniques and tools used to study the
electrophysiology of neurons.
If research scientists only focused on rats and mice as model organisms across
various science disciplines, then little progress would have been made regarding the
understanding of animal physiology.
Hodgkin and Huxley use of the giant squid axon is the most famous example of
Krogh’s principle
, The giant squid axon is a model organism in neurobiology and the starting point to
understanding AP in the NS.
in 1939, Hodgkin and Huxley inserted an intracellular electrode inside an axon and
recoded the very first AP.
In 1963, Nobel prize for physiology was awarded to Eccles, Hodgkin and Huxley for
their discoveries concerning the ionic mechanisms involved in excitation and
inhibition in the peripheral and central portions on the nerve cell membrane.
How to take the recording of the resting membrane potential of a squid giant axon?
Recording microelectrode and reference electrode are outside the axon.
Place recording microelectrode inside the squid axon
Voltmeter will start to measure inside the membrane.
Voltmeter reads inside the membrane as negative and outside as positive
RMP is -65Mv.
