Be Protein
ofINTEREST In A
patien flo For
MORE CONTROLLED EMPONMENT THERE ARE NUMEROUS
I
.
moserg
METHODS .
a
. Sonication tomog ENATE :
ofCells O
Lysig HighFrequency
: The
By G
3
1
sounds
. THIS WILL BURST THE MEMBRANE & RELEASE
THE CELL CONTENTS .
O j
Dr O
⑬
ShearingSteering O
: THE SELLS BETWEEN Thick, ·
glass
works
O O
o
d
⑬
And A
footingFung
close ER
⑧
Deter ents of a mild
detergen
The audrow ·
g
: to
SoluBMiZE THE CELL MEMBRANE
0
O
O
.
o O
Extrusion
SMALL HOLE
:
forcing
the cells
Through
THE CELL
a
very
O
T O O
TO
ESSENTIALLY TEAR APART .
2
.
Centrifugation :
Separmes MoeMes Based
forcE And
On Their >
-
DEMSMy By
Varying Centrifugal
Time
AppliED
Saltin Outiineressag
g
The coveryparous of say on THE sor row was often
- make
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CONCENTRATION As WHICH our of interest is
langer
no Soluble That
protein .
protein w r Then
Precipitate of The Solution , of
Learning
Out A solid Pellet as THE BOTTOM The Container
Fon Exchan e
g Chromatography
: Proces Are Separated
THE PROTEN OfINTEREST MUST INTERACT WIH THE MARN of THE COLUMN
By
attrge 3
. OTHER CELL CONTENTS
loter proteins of MEREST THE
By
CtAging
Wil Elite .
WE ca Release our PROTEN
PH THE BOELECTRIC
To
PON .
Size Exclusion Chromato
rapby
BASED
:
separmes proteins Ste
g
On
>
-
Tap for Sm protis
THEPORES THE allowinglegE
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point
AffinityChromatoa
Specific g hy N
:
separates protects
THE Column ProEin .
ofMTEREST Bids
based
To
on
>
-
Agand Ligand
THE In THE COLUMN WHE OTHERS ElvE . WASH WIH FREE To COLLECT
from
Target
protein column .
ofINTEREST In A
patien flo For
MORE CONTROLLED EMPONMENT THERE ARE NUMEROUS
I
.
moserg
METHODS .
a
. Sonication tomog ENATE :
ofCells O
Lysig HighFrequency
: The
By G
3
1
sounds
. THIS WILL BURST THE MEMBRANE & RELEASE
THE CELL CONTENTS .
O j
Dr O
⑬
ShearingSteering O
: THE SELLS BETWEEN Thick, ·
glass
works
O O
o
d
⑬
And A
footingFung
close ER
⑧
Deter ents of a mild
detergen
The audrow ·
g
: to
SoluBMiZE THE CELL MEMBRANE
0
O
O
.
o O
Extrusion
SMALL HOLE
:
forcing
the cells
Through
THE CELL
a
very
O
T O O
TO
ESSENTIALLY TEAR APART .
2
.
Centrifugation :
Separmes MoeMes Based
forcE And
On Their >
-
DEMSMy By
Varying Centrifugal
Time
AppliED
Saltin Outiineressag
g
The coveryparous of say on THE sor row was often
- make
protems less Soluble . one world and salt (often (NhuleSOp as the
CONCENTRATION As WHICH our of interest is
langer
no Soluble That
protein .
protein w r Then
Precipitate of The Solution , of
Learning
Out A solid Pellet as THE BOTTOM The Container
Fon Exchan e
g Chromatography
: Proces Are Separated
THE PROTEN OfINTEREST MUST INTERACT WIH THE MARN of THE COLUMN
By
attrge 3
. OTHER CELL CONTENTS
loter proteins of MEREST THE
By
CtAging
Wil Elite .
WE ca Release our PROTEN
PH THE BOELECTRIC
To
PON .
Size Exclusion Chromato
rapby
BASED
:
separmes proteins Ste
g
On
>
-
Tap for Sm protis
THEPORES THE allowinglegE
MARKATASA ,
point
AffinityChromatoa
Specific g hy N
:
separates protects
THE Column ProEin .
ofMTEREST Bids
based
To
on
>
-
Agand Ligand
THE In THE COLUMN WHE OTHERS ElvE . WASH WIH FREE To COLLECT
from
Target
protein column .
