BIOL 10016 Experiment 4 UV Spectrophotmetric Analysis of DNA (2023)(NEW)

MOHAWK COLLEGE OF APPLIED ARTS AND TECHNOLOGY
CHEMICAL ,ENVIRONMENTAL & BIOTECHNOLOGY DEPARTMENT


Lab Report
ROOM NO: FE E309


Class : BIOL 10016 Lab.

EXPERIMENT NO : 4

TITLE : UV Spectrophotometric Analysis of DNA

Submitted by :

Partners :


Instructor : Farag Soliman

Date lab performed : March 17, 2023


Date of submission : March 24, 2023




FENNELL CAMPUS
HAMILTON, ONTARIO

, Purpose

The purpose of UV spectrophotometric analysis of DNA is to accurately determine
the concentration and purity of a DNA sample. This is important for a variety of
molecular biology applications, including PCR, cloning, and sequencing. Knowing
the concentration of DNA is crucial for determining the amount of sample to use in
downstream experiments and ensuring that there is sufficient DNA for the
intended application. Additionally, measuring the purity of the DNA sample is
important because contaminants can interfere with downstream reactions,
affecting the accuracy and reliability of the results.

UV spectrophotometric analysis of DNA is a quick and convenient method to obtain
information about the quality and quantity of DNA in a sample. It is a non-
destructive technique, meaning that the DNA sample can be recovered and used in
downstream applications after the analysis. Furthermore, the equipment required
for UV spectrophotometric analysis is widely available and relatively easy to use,
making it accessible to researchers in many different fields. By providing valuable
information about the concentration and purity of a DNA sample, UV
spectrophotometric analysis is an essential tool in molecular biology research.


Procedure

Biotechnology 1 Laboratory Manual BIOL 10016 Lab. Experiment 4 UV
spectrophotometric Analysis of DNA. Pages 2-3.

Materials

Tools
 Beaker
 Micropipette
 Quartz Cuvette
 Kim Wipes

Reagents
 TBE Buffer
 Deionized Buffer
 Onion Skin Buffer

Instrument
 Spectrophotometer