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Exam (elaborations)

BIOL 439 - Quiz 2 Questions | 2026/2027 | Answers Correct | With Complete Solutions

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BIOL 439 - Quiz 2 Questions | 2026/2027 | Answers Correct | With Complete Solutions

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BIOL 439 - Quiz 2 Questions | 2026/2027 | Answers Correct |
With Complete Solutions

A blueberry farmer wants to know the concentration of
anthocyanin in a sample. Given what you know about Beer's
law, what is the most accurate concentration range of the
anthocyanin in this sample? Correct Answers Anthocyanin
does not follow typical Beer's law due to aggregate formation
meaning that although concentration increases, the number of
molecules do not. The most accurate concentration range of the
anthocyanin in this sample is 200-250 mg/mL.

A new plant species is discovered and you would like to know if
it has any allelochemicals. Briefly describe the first step you
take to purify from tissue? Correct Answers extract the tissue
using solvent (water and acetone)

Allelochemicals (algaecides) and their effects on different
processes. For example, what is their effect on photosynthesis,
effects on redox balance (list one) Correct Answers Inhibit
photosynthesis (1. abnormality of involved pigments, 2. trigger
expression changes of genes involved in photosynthesis, 3.
represses synthesis of core proteins)
- overall blocks transfer of electrons from one photosystem to
another

Redox - allelochemical increases intracellular ROS (e.g.,
H2O2). This will effect electron transport chain/enzymes.
Enzymes like Catalase (CAT) or superoxide dismutase (SOD)
used to measure the extent of ROS production

,Allelochemicals as algicides are... Correct Answers
environmentally friendly, low toxicity, selective, biodegradable,
control for harmful algae blooms (HABs)

Animals do not produce flavonoids on their own, what enzyme
are they lacking? Correct Answers Chalcone synthase

As the number of conjugated pi bonds in a molecule increases,
what happens to the corresponding wavelength of the light it
would absorb? Correct Answers the longer wavelength it
would absorb

As the number of double bonds increases, what happens to the
wavelength of light that is dissipated? Correct Answers It
increases (because it the energy needed for absorption
decreases)

Choose btw berries (ras, straw, blackberries ) - which has the
highest content of anthocyanins? Correct Answers Blackberries

Describe the negative consequences of hydrogen peroxide
production during photosensitization Correct Answers - The
production of free radicals (hydroxyl radical) as this can cause
damage to cells
- this can be detrimental to cells because hydroxy radicals can
attack any biomolecule essentially and crosslink with them and
cause damage that way

Does psoralen use a photosensitizer mechanism to kill cells?
Explain. Correct Answers Psoralen will Inhibit cell division
through the Diels-alder reaction, i.e., in other words crosslink

, two thymines (forming thymine dimers) which will disrupt the
DNA in tumor cells (targets these because they are growing out
of control)

Examples of compounds that are made toxic by UV light, don't
need to know structure but know one compound's name Correct
Answers tyrosine, tryptophan, hypericin, psoralen

Explain how flavonoids are able to generate a wide variety of
flower colors? Correct Answers Through aggregates. Each
aggregate type has a different colour.

Explain how flavonoids are able to generate a wide variety of
flower colours? Correct Answers Aggregation. Each aggregate
type has a different colour.

Explain how singlet oxygen (represented as: 1O2) is
photochemically formed and how it can damage a biological
molecule. Correct Answers Recall type 1 and type 2
photosensitization → Difference btw type 1 and 2:
- type 1 requires a substrate and type 2 does not
- the commonality is that they both lead to the formation of
radicals but type 1 requires a substrate to do so
- in the path to make the radical, type 1 requires the substrate
and type 2 only needs ground state oxygen (during rxn, go from
triplet ground state O2 to singlet O2 after PS and 3O2 react)
- 1O2 manages to damage the molecule bc it will cause the
formation of radicals which is not good for the biological system
and is damaging

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