UAMS MICROBIOLOGY FINAL EXAM
REVIEW QUESTIONS AND ANSWERS
WITH COMPLETE SOLUTIONS
ALREADY PASSED!!!
What are the key microbiological and biochemical characteristics of Listeria
monocytogenes?
✔✔ Hemolysis: Beta-hemolytic (narrow zone of beta-hemolysis on sheep blood
agar).
✔✔ Gram Stain / Morphology: Gram-positive ($+$) coccobacilli (often
resembling diphtheroids).
✔✔ Catalase: Positive ($+$).
✔✔ Bile Esculin Agar (BEA): Positive ($+$) (hydrolyzes esculin).
✔✔ CAMP Test & Hippurate: Positive ($+$).
✔✔ Motility: Motile at $25^\circ\text{C}$ (room temperature), exhibiting
characteristic "tumbling motility" in wet mounts and an "umbrella" zone of
motility in semisolid motility media.
Which notable bacterial species or genera are known to be motile at room
temperature ($25^\circ\text{C}$)?
✔✔ Listeria monocytogenes
✔✔ Yersinia enterocolitica
(Note: Both typically exhibit reduced or absent motility at $35^\circ\text{C}$–
$37^\circ\text{C}).
Which major bacterial genera are capable of producing endospores?
✔✔ Bacillus species (Aerobic or facultatively anaerobic spore-forming Gram-
positive bacilli)
✔✔ Clostridium species (Strictly anaerobic spore-forming Gram-positive bacilli)
, What are the standard incubation temperature requirements for clinical
culture media?
✔✔ Standard Pathogen Media: Incubated at $35^\circ\text{C}$ to
$37^\circ\text{C}$ (optimal for human pathogens).
✔✔ Selective Ensuing Media (e.g., CIN agar for Yersinia): Incubated at Room
Temperature (RT / $25^\circ\text{C}$) to optimize recovery and selective
isolation.
Media used to set up a wound -ANSWER✔✔BAP
MAC/EMB
PEA/CNA
Media used to set up a sputum -ANSWER✔✔BAP
MAC/EMB
CHOC
Media used to set up a body fluid -ANSWER✔✔BAP
CHOC
BROTH
Media used to set up a urine -ANSWER✔✔BAP
MAC/EMB
Media used to set up genital culture -ANSWER✔✔MTM specimens are normally
mixed with normal flora
REVIEW QUESTIONS AND ANSWERS
WITH COMPLETE SOLUTIONS
ALREADY PASSED!!!
What are the key microbiological and biochemical characteristics of Listeria
monocytogenes?
✔✔ Hemolysis: Beta-hemolytic (narrow zone of beta-hemolysis on sheep blood
agar).
✔✔ Gram Stain / Morphology: Gram-positive ($+$) coccobacilli (often
resembling diphtheroids).
✔✔ Catalase: Positive ($+$).
✔✔ Bile Esculin Agar (BEA): Positive ($+$) (hydrolyzes esculin).
✔✔ CAMP Test & Hippurate: Positive ($+$).
✔✔ Motility: Motile at $25^\circ\text{C}$ (room temperature), exhibiting
characteristic "tumbling motility" in wet mounts and an "umbrella" zone of
motility in semisolid motility media.
Which notable bacterial species or genera are known to be motile at room
temperature ($25^\circ\text{C}$)?
✔✔ Listeria monocytogenes
✔✔ Yersinia enterocolitica
(Note: Both typically exhibit reduced or absent motility at $35^\circ\text{C}$–
$37^\circ\text{C}).
Which major bacterial genera are capable of producing endospores?
✔✔ Bacillus species (Aerobic or facultatively anaerobic spore-forming Gram-
positive bacilli)
✔✔ Clostridium species (Strictly anaerobic spore-forming Gram-positive bacilli)
, What are the standard incubation temperature requirements for clinical
culture media?
✔✔ Standard Pathogen Media: Incubated at $35^\circ\text{C}$ to
$37^\circ\text{C}$ (optimal for human pathogens).
✔✔ Selective Ensuing Media (e.g., CIN agar for Yersinia): Incubated at Room
Temperature (RT / $25^\circ\text{C}$) to optimize recovery and selective
isolation.
Media used to set up a wound -ANSWER✔✔BAP
MAC/EMB
PEA/CNA
Media used to set up a sputum -ANSWER✔✔BAP
MAC/EMB
CHOC
Media used to set up a body fluid -ANSWER✔✔BAP
CHOC
BROTH
Media used to set up a urine -ANSWER✔✔BAP
MAC/EMB
Media used to set up genital culture -ANSWER✔✔MTM specimens are normally
mixed with normal flora
