Chapter 9: Microbiology: An Introduction, 13th edition

Microbiology: An Introduction, 13e (Tortora et al.)
Chapter 9 Biotechnology and DNA Technology

9.1 Multiple-Choice Questions

1) The following are steps used to make DNA fingerprints. What is the third step?
A) Collect DNA.
B) Digest with a restriction enzyme.
C) Perform electrophoresis.
D) Lyse cells.
E) Add stain.
Answer: B
Section: 9.4
Bloom's Taxonomy: Remembering
ASMcue Outcome: 8.3
Learning Outcome: 9.18




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,Figure 9.1




2) How many pieces will EcoRI produce from the plasmid shown in Figure 9.1?
A) 1
B) 2
C) 3
D) 4
E) 5
Answer: B
Section: 9.2
Bloom's Taxonomy: Analyzing
ASMcue Outcome: 4.5
Learning Outcome: 9.4
Global Outcome: 2

3) In Figure 9.1, after digestion with the appropriate restriction enzyme, what is the smallest
piece containing the entire ampicillin-resistance (amp) gene?
A) 0.17 kbp
B) 0.25 kbp
C) 1.08 kbp
D) 1.50 kbp
E) 3.00 kbp
Answer: D
Section: 9.2
Bloom's Taxonomy: Analyzing
ASMcue Outcome: 4.5
Learning Outcome: 9.4
Global Outcome: 2
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, Figure 9.2




4) In Figure 9.2, the enzyme in step 1 is
A) DNA polymerase.
B) DNA ligase.
C) RNA polymerase.
D) reverse transcriptase.
E) spliceosome.
Answer: C
Section: 9.3
Bloom's Taxonomy: Understanding
ASMcue Outcome: 4.2
Learning Outcome: 9.10




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