ASCP CG ACTUAL COMPREHENSIVE EXAM
QUESTIONS AND ANSWERS SURE A+
✔✔What chemical is used for cryopreservation? - ✔✔Dimethyl sulfoxide (DMSO)
✔✔You are analyzing a clinical case and discover a small extra marker with satellites.
What staining technique would be most useful in determining the chromosomal origin of
this marker? - ✔✔NOR
✔✔When using a fluorescein dye (yellow-green) labeled probe during the FISH
procedure, which counterstain should be used? - ✔✔Propidium iodide (red)
✔✔A bone marrow sample collected in transport medium is leaking into the sealed
plastic bag in which it was transported. The technologist should_______. - ✔✔Clean up
the tube while wearing gloves
✔✔A laboratory' quality assurance program includes _______________. -
✔✔Proficiency testing
✔✔All of the following chromosomes have dark bands on chromosome regions that can
be used as indicators when preparing the best image: - ✔✔Chromosome 11p
Chromosome 12q
Chromosome 17q
, ✔✔The morning after being set-up, a single dish of an amniotic fluid culture turns cloudy
and appears contaminated. None of the other cultures set-up that day appears
contaminated. What should be done first? - ✔✔Harvest the culture as soon as possible
✔✔When the addition of nonhomologous material to the q-arm of chromosome 16 is
suspected after G-banding, what banding technique should be used? - ✔✔C-banding
✔✔What chromosome is most different between C- and G-banding? - ✔✔Chromosome
9
✔✔What chromosome region does quinacrine and acridine orange dyes stain? - ✔✔AT
rich DNA
✔✔Under an electron microscope, what is observed as a consequence of all banding
procedures? - ✔✔Chromosomal collapse
✔✔Case #777 has an uncertain diagnosis after G and C bands. The director suspects
satellites are involved with the chrom marker in question. What banding/stain should be
used to confirm satellites are in question? - ✔✔NOR banding
✔✔What procedure should be performed first if sequential banding is required? - ✔✔Q-
banding
✔✔A tech suspects an inversion of chrom 9 with G-banding analysis. What banding
technique would confirm this? - ✔✔C-banding
✔✔What chromosome polymorphisms can be detected with Q-banding? - ✔✔Y-chrom
polymorphisms
Chrom 3 polymorphisms
Poly's of the satellites of acrocentrics
✔✔After G-banded analysis, a cyto director suspects chrom 10 has a terminal del. What
banding procedure would be MOST helpful in determining if a deletion has occurred? -
✔✔FISH
✔✔Cytogenetic studies have been requested for a patient with a diagnosis of
questionable leukemia. This patient has a low white cell count, circulating myeloblasts,
and has received chemotherapy and a blood transfusion. What procedure is LEAST
likely to be informative? - ✔✔PHA-stimulated blood culture
✔✔Special culture conditions or medium are required to demonstrate what conditions? -
✔✔Fragile X - folic acid deficient medium
QUESTIONS AND ANSWERS SURE A+
✔✔What chemical is used for cryopreservation? - ✔✔Dimethyl sulfoxide (DMSO)
✔✔You are analyzing a clinical case and discover a small extra marker with satellites.
What staining technique would be most useful in determining the chromosomal origin of
this marker? - ✔✔NOR
✔✔When using a fluorescein dye (yellow-green) labeled probe during the FISH
procedure, which counterstain should be used? - ✔✔Propidium iodide (red)
✔✔A bone marrow sample collected in transport medium is leaking into the sealed
plastic bag in which it was transported. The technologist should_______. - ✔✔Clean up
the tube while wearing gloves
✔✔A laboratory' quality assurance program includes _______________. -
✔✔Proficiency testing
✔✔All of the following chromosomes have dark bands on chromosome regions that can
be used as indicators when preparing the best image: - ✔✔Chromosome 11p
Chromosome 12q
Chromosome 17q
, ✔✔The morning after being set-up, a single dish of an amniotic fluid culture turns cloudy
and appears contaminated. None of the other cultures set-up that day appears
contaminated. What should be done first? - ✔✔Harvest the culture as soon as possible
✔✔When the addition of nonhomologous material to the q-arm of chromosome 16 is
suspected after G-banding, what banding technique should be used? - ✔✔C-banding
✔✔What chromosome is most different between C- and G-banding? - ✔✔Chromosome
9
✔✔What chromosome region does quinacrine and acridine orange dyes stain? - ✔✔AT
rich DNA
✔✔Under an electron microscope, what is observed as a consequence of all banding
procedures? - ✔✔Chromosomal collapse
✔✔Case #777 has an uncertain diagnosis after G and C bands. The director suspects
satellites are involved with the chrom marker in question. What banding/stain should be
used to confirm satellites are in question? - ✔✔NOR banding
✔✔What procedure should be performed first if sequential banding is required? - ✔✔Q-
banding
✔✔A tech suspects an inversion of chrom 9 with G-banding analysis. What banding
technique would confirm this? - ✔✔C-banding
✔✔What chromosome polymorphisms can be detected with Q-banding? - ✔✔Y-chrom
polymorphisms
Chrom 3 polymorphisms
Poly's of the satellites of acrocentrics
✔✔After G-banded analysis, a cyto director suspects chrom 10 has a terminal del. What
banding procedure would be MOST helpful in determining if a deletion has occurred? -
✔✔FISH
✔✔Cytogenetic studies have been requested for a patient with a diagnosis of
questionable leukemia. This patient has a low white cell count, circulating myeloblasts,
and has received chemotherapy and a blood transfusion. What procedure is LEAST
likely to be informative? - ✔✔PHA-stimulated blood culture
✔✔Special culture conditions or medium are required to demonstrate what conditions? -
✔✔Fragile X - folic acid deficient medium
