histology – CSMLS with correct answers 100%

histology – CSMLS with correct answers 100% stain vs dye - Correct Answer dye: coloured substance that can attach to tissue stain: alcoholic or aqueous solution of a dye 4 natural dyes - Correct Answer hema carmine saffron orcein delocalization theory - Correct Answer how benzene produces colour resonance - electrons move around chromophore; examples - Correct Answer provides colour benzene chromogen; examples - Correct Answer contains chromophore; colour in quinoid ring azo coupling nitro grouping auxochrome; examples - Correct Answer reactive group, attaches to tissue (colour stays in) amino group hydroxyl group carboxyl group sulphate group dye = - Correct Answer chromogen + auxochrome which auxochromes are positively charged - Correct Answer amino group which auxochromes are negatively charged - Correct Answer carboxyl hydroxyl sulphate a positive auxochrome produces a __________ (cationic/anionic) and _________ (acidic/basic) dye - Correct Answer cationic; basic most acid/basic stains are _______ - Correct Answer salts anionic dye acidic/basic? acidic component? basic component? auxochrome? tissue? dye charge? - Correct Answer acidic coloured colourless neg pos (acidophillic) neg pos tissue examples - Correct Answer cytoplasm, RBCs, muscle, collagen neg tissue examples - Correct Answer DNA, chromatin, RNA, mucus, lipids examples of anionic dyes - Correct Answer eosin, saffron, aniline blue, light green, biebrich acid fuschin examples of cationic dyes - Correct Answer hematoxylin what type of dyes are dissolved in methanol - Correct Answer neutral neutral dye where is the chromophore - Correct Answer both the cationic and anionic component have a chromophore neutral dye examples - Correct Answer romanowsky stain: methylene blue (cationic) + eosin (anionic)