Microbio 150 Lab exam 1 PVCC-
Questions & Answers
When preparing to view a specimen on a compound microscope in the lab, one should
__________ begin with the 4x objective Correct Ans-Always
Where should used gloves be disposed of in the microbiology lab Correct Ans-In the bio-
hazard waste container
Before putting a dust cover over the microscope and placing it in the cabinet, which objective
lens should be rotated over the stage Correct Ans-4x
On the microscope, what concentrates the light on the specimen? Correct Ans-Condenser lens
On the microscope, what bends light passing through the specimen to create a real image
Correct Ans-Objective lens
On the microscope, what allows for more or less light to be directed onto the specimen
Correct Ans-Iris diaphragm
On the microscope, what gives you clarity of the image Correct Ans-Resolution
, Why is desiccating your inoculating loop important? When should this be done? Correct Ans-
To ensure it is sterilized, done so no cross contamination happens
During an aseptic transfer, when do you pass the mouth of the test tube through the flame?
Correct Ans-Before and after dipping loop in
During the broth to slide aseptic transfer, a __________ was used to obtain a pure culture from a
__________. Correct Ans-Loop, broth
Prior to staining, smears of microorganisms are heat-fixed in order to Correct Ans-Attach it
firmly to the slide
The term that refers to the purposeful addition of microorganisms into a nutrient medium is
Correct Ans-Inoculation
True or false: In the microbiology lab, it is acceptable to move your loop around while waiting
for it to dry. Correct Ans-False
Which stain was used during the negative staining procedure? Correct Ans-Nigrosin
Which stain was used during the simple staining procedure? Correct Ans-Methylene blue
True or false: During a negative stain, the bacteria are heat fixed. Correct Ans-False
Questions & Answers
When preparing to view a specimen on a compound microscope in the lab, one should
__________ begin with the 4x objective Correct Ans-Always
Where should used gloves be disposed of in the microbiology lab Correct Ans-In the bio-
hazard waste container
Before putting a dust cover over the microscope and placing it in the cabinet, which objective
lens should be rotated over the stage Correct Ans-4x
On the microscope, what concentrates the light on the specimen? Correct Ans-Condenser lens
On the microscope, what bends light passing through the specimen to create a real image
Correct Ans-Objective lens
On the microscope, what allows for more or less light to be directed onto the specimen
Correct Ans-Iris diaphragm
On the microscope, what gives you clarity of the image Correct Ans-Resolution
, Why is desiccating your inoculating loop important? When should this be done? Correct Ans-
To ensure it is sterilized, done so no cross contamination happens
During an aseptic transfer, when do you pass the mouth of the test tube through the flame?
Correct Ans-Before and after dipping loop in
During the broth to slide aseptic transfer, a __________ was used to obtain a pure culture from a
__________. Correct Ans-Loop, broth
Prior to staining, smears of microorganisms are heat-fixed in order to Correct Ans-Attach it
firmly to the slide
The term that refers to the purposeful addition of microorganisms into a nutrient medium is
Correct Ans-Inoculation
True or false: In the microbiology lab, it is acceptable to move your loop around while waiting
for it to dry. Correct Ans-False
Which stain was used during the negative staining procedure? Correct Ans-Nigrosin
Which stain was used during the simple staining procedure? Correct Ans-Methylene blue
True or false: During a negative stain, the bacteria are heat fixed. Correct Ans-False
