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Microbiology Lab – Stains Exam Questions And Answers 100% Pass.

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Microbiology Lab – Stains Exam Questions And Answers 100% Pass. Simple stain - Answer-Add color to cells for viewing -Highlight entire organism's shape and basic structures -Includes: Methylene blue Safranin Crystal violet Carbolfuchsin Negative stain - Answer-Enhances viewing of morphology, capsules -Bacteria are live -No heat fixing! (no denaturing) -Nigrosin ink -View under oil -Anionic (acidic) simple stain Gram stain - Answer-Determines cell wall type (gram positive or negative) -Differential stain Gram stain procedure - Answer1. Crystal violet 2. Iodine (mordant) 3. Alcohol (decolorizer) 4. Safranin (counterstain) 5. Observe under oil Mordant - gram stain - Answer-Increases intensity of crystal violet -Iodine enters cell forms crystal violet-iodine complex (CV-I)

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2024/2025
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  • microbiology lab stains exam questions and answe

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Microbiology Lab – Stains Exam Questions
And Answers 100% Pass.


Simple stain - Answer✔-Add color to cells for viewing
-Highlight entire organism's shape and basic structures
-Includes:
Methylene blue
Safranin
Crystal violet
Carbolfuchsin
Negative stain - Answer✔-Enhances viewing of morphology, capsules
-Bacteria are live
-No heat fixing! (no denaturing)
-Nigrosin ink
-View under oil
-Anionic (acidic) simple stain
Gram stain - Answer✔-Determines cell wall type (gram positive or negative)
-Differential stain
Gram stain procedure - Answer✔1. Crystal violet
2. Iodine (mordant)
3. Alcohol (decolorizer)
4. Safranin (counterstain)
5. Observe under oil
Mordant - gram stain - Answer✔-Increases intensity of crystal violet
-Iodine enters cell forms crystal violet-iodine complex (CV-I)
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