Blood Bank Techniques Exam Questions with complete Solutions 100%

Blood Bank Techniques Exam Questions with complete Solutions 100% IAT - Answers Antihuman Globulin testing /Coomb's testing DAT - Answers 1 step process: AHG For AIHA, DIHA, HDN, HTR IAT - Answers IN VITRO sensitization of red cells DAT - Answers IN VIVO sensitization of red cells IAT - Answers 2 steps process. For ANTIBODY DETECTION, ANTIBODY IDENTIFICATION, ANTIBODY TITRATION, RBC PHENOTYPE Compatiblity testing, Antibody Screening - Answers Tests for ANTIBODY DETECTION Compatibility testing - Answers RECIPIENT Antibody with DONOR cells Antibody screening - Answers Antibody reacting with SCREENING cells Antibody panel - Answers Antibody reacting with PANEL cells Antibody panel - Answers A test for ANTIBODY IDENTIFICATION Rh Antibody titer - Answers A test for ANTIBODY TITRATION RBC antigen detection - Answers A test for RBC PHENOTYPE Antibody panel - Answers Antibody reacting with PANEL cells Antibody titration - Answers Antibody and selected Rh cells Rbc antigen detection - Answers Specific antisera+ RBCs to detect antigen Allows time for antibody molecule attachment to RBC antigen - Answers Purpose of incubating RBC with Antisera. In IAT Removes free globulin molecules - Answers Purpose of performing minimum of 3 saline machine To form RBC Agglutinate - Answers Purpose of adding AHG REAGENT Centrifuge - Answers Acceletrates agglutination by BRINGING CELLS TOGETHER Positive Negative - Answers In IAT, if there is agglutination interpret as To determine the strength of reaction - Answers Why do you need to GRADE AGGLUTINATION REACTION Check cells - Answers CHECKS for NEUTRALIZATION of ANTISERA by free globulin molecules. Check cells - Answers IgG sensitized RBC, (+) agglutination (-) invalid 40:1 - Answers Serum to cell ratio 133:1 - Answers Serum to cell ratio when detecting weak Antibodies Adding 2 drops serum 1 drop 5% RCS - Answers Achieve 40:1 minimum ratio by Adding 4 drops serum 1 drop % RCS - Answers Achieve 133:1 ratio in weak antibody detection Albumin LISS PEG - Answers Reaction medium in AHG test Albumin - Answers A reaction medium that allow antibody coated cells to come into closer to each other LISS - Answers Increase antibody uptake, allow incubation time to decrease from 30-60 mins to 10-15 mins by reducing ZETA POTENTIAL. Zeta potential - Answers Reduces incubation time in LISS PEG - Answers Water soluble linear polymer used as an additive to increase Antibody uptake by REMOVING WATER MOLECULES surrounding the RBC thereby concentrating the antibody ABO RH typing Antibody screening Crossmatch - Answers Compatibility tesitng for HOMOLOGOUS transfusion Unexpected antibodies - Answers Screening of the donor's and recipient serum for___ ABO Rh typing Antibody screening - Answers Compatibility testing for AUTOLOGOUS transfusion Autologous transfusion Neonatal transfusion - Answers Crossmatch NOT required O- - Answers In cases of EMERGENCY. What blood should be given Transfuse 8-10 units in less than 24 hours or 4-5 units in 1 hour - Answers Massive transfusion Crossmatch - Answers Final check of ABO compatibility. May detect presence of antibody in patient's serum that will react with antigens on donor's RBC that was Not detected in antibody screening Immediate spin crossmatch - Answers Mix PSDR and centrifuge immediately. NO clinical significant unexpected antibodies detected and NO previous record of antibodies Type and screen - Answers Testing patient blood sample for ABO, Rh. Clinically significant unexpected antibodies. Patient sample then stored in BB ref for future crossmatch if blood is needed for transfusion. Abbreviated crossmatch - Answers Immediate spin + type and screen Antiglobulin crossmatch - Answers A crossmatch that Begins the sane manner as immediate spin crossmatch. Continue at 37C incubation, Finishes with antiglobulin test Computer crossmatch - Answers Compares Recent ABO results on file for bothdonor and recipient Computer crossmatch - Answers Can only be used for purpose of detecting ABO incompatibility Blood spectrum compatibility test - Answers Method of choice for crossmatch