BIOS260 Chapter 9 Questions and Answers 100% Solved
What substrates are used in the DNA synthesis reaction? Correct Ans-Deoxyadenosine
triphosphate
Deoxyguanosine triphosphate
Deoxycytosine triphosphate
Deoxythymidine triphosphate
List the different proteins and enzymes taking part in bacterial replication. Give the function
of each in the replication process. Correct Ans-1.Initiator proteins
bind to the origin and separates strands of DNA to initiate replication.
2. DNA helicase
unwinds the double helix by breaking the hydrogen bonding between the 2 strands at the
replication fork.
-pushes forks open to make a bubble
3. Single-stranded binding protein (SSB protein) stabilizes single-stranded DNA prior to
replication by binding to it, thus preventing the DNA from pairing with complementary
sequences.
, BIOS260 Chapter 9 Questions and Answers 100% Solved
OTHER WORDS- attach to single stranded DNA and prevent secondary structures from
forming.
-holds bubble open
4.DNA gyrase
-moves ahead of the replication fork, making and resealing breaks in the double-stranded
helical DNA to release the torque that builds up as well as a result of unwinding at the
replication fork
-untwists DNA
5. DNA primase
Specific for DNA
an RNA polymerase
Synthesizes a short RNA primer to provide a 3'-OH group for the attachment of DNA
nucleotides
6. DNA polymerase III
What substrates are used in the DNA synthesis reaction? Correct Ans-Deoxyadenosine
triphosphate
Deoxyguanosine triphosphate
Deoxycytosine triphosphate
Deoxythymidine triphosphate
List the different proteins and enzymes taking part in bacterial replication. Give the function
of each in the replication process. Correct Ans-1.Initiator proteins
bind to the origin and separates strands of DNA to initiate replication.
2. DNA helicase
unwinds the double helix by breaking the hydrogen bonding between the 2 strands at the
replication fork.
-pushes forks open to make a bubble
3. Single-stranded binding protein (SSB protein) stabilizes single-stranded DNA prior to
replication by binding to it, thus preventing the DNA from pairing with complementary
sequences.
, BIOS260 Chapter 9 Questions and Answers 100% Solved
OTHER WORDS- attach to single stranded DNA and prevent secondary structures from
forming.
-holds bubble open
4.DNA gyrase
-moves ahead of the replication fork, making and resealing breaks in the double-stranded
helical DNA to release the torque that builds up as well as a result of unwinding at the
replication fork
-untwists DNA
5. DNA primase
Specific for DNA
an RNA polymerase
Synthesizes a short RNA primer to provide a 3'-OH group for the attachment of DNA
nucleotides
6. DNA polymerase III
