Blood Bank DAT/IAT questions and answers 100% correct

Blood Bank DAT/IAT questions and answers 100% correct This test is also known as the antiglobulin test Coombs test The _______ ________ test is used to detect antibodies (IgG or C3) that are stuck to the surface of RBCs. Many diseases and drugs can cause this. These antibodies sometimes destroy red cells and cause anemia. Direct Coombs Test (DAT ) This is the test that is done on newborn's blood sample, usually in the setting w/ a newborn w/ jaundice. The 2 most commonly recognized forms of antibody-mediated hemolysis in newborns are Rh incompatibility and ABO incompatibility. The Direct Coomb's Test or DAT Detects antibodies o complement bound to the surface of RBCs In-Vivo DAT Washed & incubated w/ AHG and agglutination = Positive Is it DAT or IAT? DAT Used to detect antibody-antigen reaction In-Vitro. IAT (Indirect Coombs Test) The __________ ________ test looks for fee-flowing antibodies against certain RBCs. It is most often done to determine if you may have a reaction to a blood transfusion. This test is also done on the mother's blood sample as part of her prenatal labs. Frequently referred to as the "antibody screen", this test identifies a long list of minor antigens that could either cause problems in the newborns or cause problems in the mother if transfusion is necessary. IAT (Indirect Coombs test/antiglobulin test) Clumping (agglutination) duing the __________ coomb's test means that you have antibodies on the RBCs and that you may have a condition that causes the destruction of RBCs by you immune system (hemolysis). This may be due to Hemolytic anemia CLL or similar disorder Erythroblastosis fetalis (hemolytic disease of the newborn) The Direct Coombs Test (DAT) Antibodies reacting in this phase are considered clinically significant. They re usually Warm reactive and IgG. (IgG: Grandma is Warm) AHG Antibodies in this phase tend to be cold reactive. They are usually IgM and NOT clinically significant (with the exception of A and B antibodies) (IgM: Mother is Cold) Immediate spin (IS phase) Antibodies that react in this phase include strong IgM or IgG antibodies. After incubation the tubes are examined for the presence of hemolysis . If complement was bound during incubation that hemolysis would be seen. 37 degrees Celsius (37C) Antibodies in the Duffy, Kidd, and Kell blood group systems (anti-Fya, anti-Jk, anti-K) react most commonly in which phase of antibody identification? AHG