Portage Microbiology Exam 4 Questions and Answers
Question 1
True or False: Growth media is best suited for distinguishing between two similar species of bacteria
Correct Answer
False. Growth media is designed to simply support (and not restrict) microbial growth
Question 2
A researcher is asked to determine which of two vials contains e coli and which contains salmonella. Knowing both gram-
negative while only one of them is capable of fermenting lactose, which type of media would be best suited (multiple
choice)
Correct Answer
Differential media distinguishes between two, often related, microbes
Question 3
A researcher is asked to determine if a sample contains Neisseria meningitides. Knowing Neisseria meningitides is slow
growing and other foreign microbes may also be present in the culture, which type of media would be best suited?
(multiple choice)
Correct Answer
Selective media
Page 1 of 10
, Question 4
What are the requirements of a fastidious microbe? (short answer)
Correct Answer
A fastidious microbe is an organism with complex growth requirements such that is absent it will not grow. Enriched
medias thus contain these specific and essential nutrients required for the growth of a particular subset of
microorganisms.
Question 5
True or False: LB agar is classified as a selective, non-differential media
Correct Answer
False
Question 6
What is agar used for in microbiology? (short answer)
Correct Answer
Agar is used to create a solid, smooth surface on which microbes can grow
Question 7
Alpha hemolysis (matching)
Correct Answer
Incomplete hemolytic activity
Page 2 of 10
Question 1
True or False: Growth media is best suited for distinguishing between two similar species of bacteria
Correct Answer
False. Growth media is designed to simply support (and not restrict) microbial growth
Question 2
A researcher is asked to determine which of two vials contains e coli and which contains salmonella. Knowing both gram-
negative while only one of them is capable of fermenting lactose, which type of media would be best suited (multiple
choice)
Correct Answer
Differential media distinguishes between two, often related, microbes
Question 3
A researcher is asked to determine if a sample contains Neisseria meningitides. Knowing Neisseria meningitides is slow
growing and other foreign microbes may also be present in the culture, which type of media would be best suited?
(multiple choice)
Correct Answer
Selective media
Page 1 of 10
, Question 4
What are the requirements of a fastidious microbe? (short answer)
Correct Answer
A fastidious microbe is an organism with complex growth requirements such that is absent it will not grow. Enriched
medias thus contain these specific and essential nutrients required for the growth of a particular subset of
microorganisms.
Question 5
True or False: LB agar is classified as a selective, non-differential media
Correct Answer
False
Question 6
What is agar used for in microbiology? (short answer)
Correct Answer
Agar is used to create a solid, smooth surface on which microbes can grow
Question 7
Alpha hemolysis (matching)
Correct Answer
Incomplete hemolytic activity
Page 2 of 10
