bio 171 Lab 7 Notebook

bio 171 Lab 7 Notebook. Detailed working, solutions, memo, notes & explanations. Lab 7 Notebook Back to Home Page Title: PL#7 Secondary Assays for Bacterial Characterization Objective: This lab identifies the profile of bacterial populations that are based on additional biochemical approaches to best determine the growth and metabolic characteristics. Indole Test: Biochemical test to determine a bacterial species ability to convert (breakdown) tryptophan into indole. Procedure: Test #1 1. Begin by using a sterile loop to pick an isolated colony from BAP 2. The next step is to spread it directly onto the reaction site of the slide. 3. Examine test area for color change within 20 seconds Note: If the Indole is negative, that would equal yellow in color If the Indole is positive that would equal a Red and Pink orange color Test #2 1. Use a sterile loop to pick an isolated colony from the BAP 2. The you need to Inoculate the bacteria in media broth 3. Grow at 37 degrees Celsius overnight 4. Then add 5 drops of Kovac’s reagent to the culture Note: If the Indole is negative it would be a yellow color If the Indole is positive it will equal a Red and Pink/orange color TSI Test: Triple Sugar Iron agar is used to evaluate the ability of bacteria to ferment sugars and/or to produce hydrogen sulfide. 1. Using a sterile loop, inoculate slant from the liquid culture 2. Then it is necessary to press the loop into the agar slant 3. When removing loop, smear slanted side of agar with the loop 4. Grow overnight at 37 degrees Celsius