(MB) ASCP Practice Exam Questions and Answers

(MB) ASCP Practice Exam Questions and Answers Which of the following is not a component of a nucleotide? Phosphate group Anti-codon Ribose sugar Nitrogen base - ANSWER Anti-codon According to Chargaff's rule of base pairing, adenine pairs with: - ANSWER Thymine What genes would be screened in a breast cancer panel? - ANSWER HER2, ERBB2, BRCA1 Next Generation Sequencing uses: - ANSWER Short sequence reads Purines and pyrimidines differ from each other in that: - ANSWER Purines have two rings; pyrimidines have one ring The purines are: Cytosine and uracil Adenine and thymine Thymine and cytosine Adenine and guanine - ANSWER Adenine and guanine What is the rate of mutation per round of DNA replication? 1 in 1,000 base pairs 1 in 10,000 base pairs 1 in 1,000,000 base pairs 1 in 1,000,000,000 base pairs - ANSWER 1 in 1,000,000,000 base pairs The rate of DNA migration through an agarose gel during electrophoresis does not depend on which of the following factors? Net charge of the molecule Size of the molecule Shape of the molecule Nucleotide sequence of the molecule - ANSWER Nucleotide sequence of the molecule What are the phases in a qPCR Amplification Plot? Initiation, exponential, plateau Baseline, exponential, plateau Baseline, threshold, exponential, plateau Baseline, initiation, threshold, exponential, plateau - ANSWER Initiation, exponential, plateau Find the palindrome in this restriction enzyme site: 5'-CTGCAG-3'? 5'-GAC 3'-GAC 3'-CTG 5'-GTC - ANSWER 3'-GAC