Demetrius

What does TSI stand for? correct answer: Triple Sugar Iron How do you inoculate a TSI? correct answer: Stab and Drag What does a TSI slant agar select for? correct answer: Differentiates between non-fermenters, glucose fermenters and fermenters of lactose and/or sucrose. It is used to identify intestinal bacteria. What does a black medium on SIM agar indicate? correct answer: Positive for sulfur reduction and H₂S production What agent do you add to the indole test and what orga...

Why is the catalytic activity of enzymes essential to ensure and regulate cellular metabolism? correct answer: enzymes lower the activation energy for chemical rxn. Why are microorganisms able to cause diary products, such as milk, to sour or curdle? correct answer: microorganisms can break down the nutrients in the milk and cause it to sour or curdle. Give a reason why it is necessary for polysaccharides, such as starch or cellulose, to be digested outside of the cell even though di-sac...

*Selective:* Selects for: Gram (+) *Components:* Phenylethyl alcohol (used for isolation of Gram + from mixed cultures) Plate A is a PEA plate with a Gram positive organism on the left and a Gram negative organism on the right. Plate B is a nutrient agar plate illustrating normal growth of each of the same bacteria. Notice that the Gram-negative organism shows inhibited growth on the PEA plate. Phenylethyl Alcohol agar (PEA/PAA) Phenylethanol Agar (PEA) Phenylethanol inhibits many Gr...

TSI Agar correct answer: *stab and drag *yellow= acid production/ fermentaion of the sugars in the slant/butt. *red= negative *black= H2S production (salmonella or proteus) what are we looking for in the Indole test after adding kovacs reagant? correct answer: * a red ring which indicates that the amino acid tryptophan has been degraded into indole. Methyl red test results? correct answer: red= lots of acid (PH of 4) normally yellow=acidic Simmons citrate correct answer: to see i...

Organisms that require specialized environments due to complex nutritional requirements are known as...? correct answer: fastidious organisms The blood agar plate (BAP) is an enrichment medium that tests for fastidious organisms such as? correct answer: Streptococcus spp. (species) The blood agar permits demonstration of what property of microbes, such as streptococci...? correct answer: hemolytic properties The blood agar test can show what three possible hemolytic results...? corre...

FeCl3 Used in a PAA agar to detect presence of phenylpyruvic acid positive green color. MAC agar What stain do you do on Day 1 of the unknown lab? You may Gram stain from your unknown broth to confirm if the bacteria are Gram-positive or Gram-negative. A throat culture performed by using a throat swab is used to detect which organism that causes strep throat? Group A Streptococcus Bacteria Which staphylococci is the most important bacteria in human disease? Staphylococcus Aureus W...

Phenylethyl alcohol agar PAA correct answer: Selective media- used to isolate specific groups of bacteria Isolation of mostly Gram positive organisms ** Phenylethyl alcohol ** inhibits Gram negative organisms colonies may be visible , but smaller in number and size Phenylethyl alcohol agar Media Test Reading correct answer: Lab results: S. aureus : Amount of growth (1 + ) Appearance of growth ( light golden ) Appearance of medium (----------) E. faecalis : Amount of grow...

TSI stands for correct answer: Triple Sugar Iron If peptone is metabolized then what is made correct answer: Ammonia What are the 3 carbohydrates present for TSI correct answer: Glucose Sucrose Lactose Glucose is a correct answer: monosaccharide Sucrose and Lactose are correct answer: disaccharides Monosaccharides differ from disaccharides by correct answer: using less energy to be broken down Ph indicator that is used is correct answer: Phenol Red The color of the ag...

What sugars does triple sugar iron agar contain? correct answer: 1% Lactose 1% Sucrose 0.1% Glucose (Dextrose) What is TSI used for? correct answer: To differentiate enterics based on the ability to reduce sulfur and ferment carbohydrates What is fermented with a red (alkaline) slant and a yellow (acid) deep? (K/A) correct answer: Only glucose What is fermented with a yellow (acid) slant and a yellow (acid) deep? correct answer: Glucose and Lactose and/or Sucrose What is fermen...

TSI stands for________________________? correct answer: Triple Sugar Iron What is the sulfur source in TSI agar? correct answer: Sodium thiosulfate A red slant and a red butt with TSI agar indicates that the microorganism ________? correct answer: Cannot ferment A red slant and a yellow butt with TSI agar indicates that the microorganism ________________________________? correct answer: Ferments only glucose Why must TSI agar be read, 24 hours after inoculation? correct answer: ...

If there are 2.2 pounds in 1 kilogram, how many pounds are there in x kilograms? correct answer: 2.2x The bar graph above shows the number of customers who stopped at a store Monday through Thursday of one week. If the number of customers on Friday was a one-fifth increase over the number of customers on Thursday, how many customers shopped at the store on Friday? correct answer: 480 Last year, a bakery sold w loaves of bread. This year, the bakery sold three more than twice the number o...

In 2010, talk show host Oprah Winfrey and novelist Jonathan Franzen kissed and made up after a nine-year feud. In 2001, Franzen was disinvited from appearing on Winfrey's TV show to pitch his novel The Corrections after he made it clear that he was unhappy about the book's being chosen for the Oprah Book Club. Describing his work as "in the high-art literary tradition," Franzen said he didn't want to be associated with the Club, which he accused of occasionally choosing "schmaltzy, one-di...

The microwave's buzzer sounded dinner was ready. a. The microwave sounded dinner was ready b. The microwave sounded, dinner was ready. c. The microwave sounded since dinner was ready. d. The microwave sounded, since dinner was ready. correct answer: c. The microwave sounded since dinner was ready. By setting aside time to study everyday, a grade point average is sure to improve. a. By setting aside time to study everyday, a grade point average is sure to improve. b. By setting asi...

What Act, passed by Congress after Sept. 11, 2001, established the Transportation Security Administration? correct answer: Aviation and Transportation Security Act What Act, passed as the first Act of the 110th Congress, established 100% cargo screening and spelled out specific staffing levels for Surface Inspectors? correct answer: Implementing Reccomendations of the 9/11 Commission Act of 2007 TSR means the regulations issued by...(1500.3) correct answer: the Transportation Security A...

For what bacteria would you use the TSI test? correct answer: Gram negative bacilli such as enterobacteriaceae. How would you identify an acidic slant? correct answer: If the slant goes from red to yellow on the phenol red. Why is there more lactose and sucrose in TSI agar than glucose? correct answer: In order to distinguish bacteria that can only ferment glucose from bacteria that ferment lactose and sucrose. It will also allow for greater acid production. TEST RESULTS: red slant/r...

There is no evidence that mermaids do exist. However, theories are still being made in an attempt to prove this 'false'. The belief in mermaids may have arisen at the very dawn of our species. Magical female figures first appear in cave paintings in the late Paleolithic (Stone Age) period some 30,000 years ago, when modern humans gained dominion over the land and, presumably, began to sail the seas. Half-human creatures, called chimeras, also abounded in mythology We can infer from the pass...

What does TSI mean? correct answer: Triple Sugar Iron What is a TSI (Triple Sugar Iron) Test also referred to as? correct answer: Kligers Iron Test What does a TSI Test, test for? correct answer: Bacteria's ability to: -Ferment glucose and lactose/sucrose -Produce gas -Reduce Sulfur What are the ingredients of this media? correct answer: Glucose, Sucrose, and Lactose What is the ratio of Glucose, Sucrose, and Lactose in this media? correct answer: 1:10:10 What is the sulf...

List the tests that are involved with TSI. correct answer: H2S Gas and Carbohydrate Fermentation What is the media for H2S Gas? correct answer: TSI agar What method do you use to place content on the media? correct answer: stab and drag motion what is the substrate for H2S gas? correct answer: cystein What is the product for H2S gas? correct answer: H2S What is the reagent for H2S gas? correct answer: iron in media What is the pH indicator for H2S gas? correct answer: no...

PEMDAS correct answer: (parenthesis, exponents, multiplication, division, addition, subtraction) work left to right Exponential Notation correct answer: Ex: 6^4, 2^3, 9^10, etc. Expanded Form correct answer: Ex: 7x7x7, 6x6, 2x2x2x2x2, etc. Standard Notation correct answer: Ex: 5^2= 25; 25 is the standard notation (the answer or solution) +x+ correct answer: =+ +x- correct answer: =- -x- correct answer: =+ Adding or Subtracting fractions correct answer: You need a com...

Is TSI gram+ or gram- correct answer: gram- What is the application of TSI? correct answer: used to differentiate members of Enterobacteriacea and to distinguish them from other gram- rods What is the media in TSI (triple sugar iron) correct answer: 1 glucose, lactose & sucrose 2 animal protein 3 ferrous sulfate 4 sodium thiosulfate 5 phenol red What is the purpose of glucose, lactose & sucrose correct answer: carbohydrate for fermentation What is the purpose of animal proteins? ...