Experimental Cell Biology 2 (AB_1048)

Experimental Cell Biology II 2022-2023

This document provides a summary of the whole Experimental Cell Biology 2 course. **You can also find the separate lectures on my profile! Topics that are discussed are: Endothelial cells (leukocyte extravasation, chemokines, and stem cell homing), Basic techniques in cell biology (PCR, chromatography, sequencing, knock-out, fluorescent probes, RNAi, and CRISPR/Cas9), Proteomics/ mass spectrometry (including Alzheimer’s, LCM, tangle formation and braak stages), Host factors (Salmonella, S...

This document provides a summary of proteomic techniques, such as mass spectrometry. The focus lies on tandem MS, peptide fragmentation, MS/MS, Maldi and electrospray, Time-of-flight, orbitrap and the pathology of Alzheimer’s disease and how the MS contributes to the research. There is also a brief summary of Braak stages, laser capture microscopy, tangle formation, and immunohistochemistry.

This document provides a summary of the lecture Single-cell gene expression. Topics that are discussed are: mRNA, mRNA expression, Northern blot, qPCR, RNA sequencing, DNA microarray, gene expression, single-cell gene expression analysis, High-throughput single-cell RNA analysis methods, Low throughput single-cell RNA analysis methods, FISH, smFISH, mRNA localization, Gene expression analysis in living cells and MS2/PP7 systems

This document contains lecture notes of the entire course. It contains images with descriptions of them when necessary.

This document contains an overview of the discovery and pathway of apoptosis. In this summary literature articles are used and linked.