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PCB 3134 Final Exam – Questions With Indepth Solutions

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PCB 3134 Final Exam – Questions With Indepth Solutions

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PCB 3134 Final Exam – Questions With Indepth Solutions

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Practice questions for this set


Learn 1 /7 Study with Learn




Exist in cells to guide the proper folding of polypeptide (Hsp70 and Hsp60)



Choose an answer



The sequence of the template DNA strand
1 Molecular Chaperones 2 is 5'-CTGA-3'. What is the sequence of the
mRNA?



3 Pre-mRNA 4 Pol I makes:



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Terms in this set (112)


The sequence of the template 5'-UCAG-3'
DNA strand is 5'-CTGA-3'. What
is the sequence of the mRNA?

the DNA strand that has the SAME nucleotide order as the
Coding Strand
mRNA from 5' to 3'

the actual strand that is used as the template for RNA synthesis
Template Strand
(non-coding) and is complementary to the mRNA

1. Binding
2. Initiation
Four steps of Transcription
3. Elongation
4. Termination

Pol I makes: most rRNAs - promoter upstream

Pol II makes: mRNA, snRNA and niRNA - promoter upstream

tRNA and one type of rRNA - promoter is within the transcription
Pol III makes:
unit

TFIID TATA box binding

, Helicase- melts promoter region
TFIIH
Kinase-phosphorylates Pol II

Transcription by pol I is a protein that recognizes an 18-nucleotide signal in the growing
terminated by: RNA chain

Transcription of Pol III is termination signals include short run of Us and no protein facts
terminated by: are required

Transcription of Pol II os cleavage at a specific site before transcription ceases (10-35
terminated by: nucleotides downstream)

crude produce of Pol II transcription, needs further processing
Pre-mRNA
to be mRNA (poly A tail, 5' cap, splicing)

Polyadenylation addition of a poly A tail to the 3' end of mRNA strand

addition of a 5' cap to the 5' end. Note the 5'-5' linkage. Adds
5' capping
stability and ribosome position

Splicing removal of introns and connection of the exons

use of alternative axons generates mRNAs that encode different
Alternative Splicing
iso forms of a protein. Use the same RNA for multiple purposes

Carry out reverse transcriptase (RNA-DNA). Integrate DNA into
Retrovirus host genome (Provirus). Transcription of provirus to make more
viral RNA (DNA-RNA)

T/F Processing of rRNA is the False - rRNA does not combine after spacers are removed
same as processing of mRNA

APOBEC3G edits the HIV genome to dehabilitate a virus

Vif protein An HIV protein that counteracts APOBEC3G

miRNA regulate gene expression mainly by suppressing translation

1. Triplet Code with start and stop codons
The Genetic Code is: 2. Nonoverlapping
3. Degenerate

A stretch of DNA without any stop codon. (usually only part of
Open reading frame
the mRNA)

Sickle cell anemia caused by a single mutation in the B hemoglobin gene (HBB)

unusual base pairing can be formed at the THIRD position of the
Wobble Hypothesis
codon

link amino acids to correct tRNA. ATP driven reaction to link A.A
Aminoacyl-tRNA synthetase
to tRNA and high energy ester bond formed

AUG needed.
1. Methionyl tRNA bound by eIFs
2. Methionyl tRNA bind to the small subunit
Initiation
3. This binds to the 5'cap of the mRNA
4. Find Kozak sequence
5. tRNA base-pairs with start codon, then large subunit

Elongation sequential cycles and is facilitated by elongation factors

done by reaching a stop codon and causes a release factor to
Termination
separation

A nonsense mutation is one that B. Premature termination of translation
causes
A. incorporation of the wrong
amino acid at one position in a
polypeptide
B. premature termination of
translation
C. either A or B

lead to an incomplete, nonfunctional polypeptide (premature
Nonsense Mutations
stop codon formed)
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