BCHS 3201 LAB PRELAB QUESTIONS AND ANSWERS |
2025/2026 UPDATE | 100% CORRECT
The study of all of the proteins expressed in an organism at a particular time
and/or set of conditions is called: Answer - Proteomics
In a microarray, to see what genes have been expressed (transcribed) by the
cell under our set of experimental conditions, we will purify ___________ from
the organism: Answer - mRNA
For our microarray project, the model organism under study is: Answer -
Arabidopsis thaliana
How is the protective group removed to allow the addition of nucleotides to
the probe on the DNA chip? Answer - UV light exposure
What is in a probe pair on an Affymetrix chip? Answer - One probe to the
specified gene and one probe with a mismatch in the center of the probe to
gauge non-specific hybridization levels
In the yeast microarray example, which genes are downregulated when the
yeast are grown on minimal media instead of rich media? Answer - c, d
Why are side groups on a nucleotide capped when building probes on a
microarray? Answer - To prevent elongation of probes that have incorporated
a nucleotide in the wrong place
, What plant phytohormone inhibits seed germination to prevent premature
sprouting? Answer - Abscisic acid
In which processes is sugar signaling involved? Answer - Starch metabolism,
Flowering, Heat senescence, Seed germination
If sugar levels in a plant are high, you would expect expression of genes
involved in photosynthesis to be: Answer - Downregulated
Which of the following represents a properly balanced centrifuge (check all
that apply): Answer - a
How will the plasmid DNA be separated from chromosomal DNA in this plasmid
purification lab? Answer - Differential centrifugation
What happens to plasmid DNA if you leave it in Solution II, lysis buffer, for too
long? Answer - DNA Degrades
After lysing your E. coli cells and neutralizing the reaction, do you keep the
supernatant or pellet for the next step? Answer - Supernatant
Which has more resolving power? Answer - 4% agarose gel
What should you do with the liquid waste from this lab? Answer - Bleach it for
20 minutes
Why must you wait 10 minutes after adding TE/RNase to the dry DNA pellet?
Answer - To allow time for the RNA to be hydrolyzed or degraded
2025/2026 UPDATE | 100% CORRECT
The study of all of the proteins expressed in an organism at a particular time
and/or set of conditions is called: Answer - Proteomics
In a microarray, to see what genes have been expressed (transcribed) by the
cell under our set of experimental conditions, we will purify ___________ from
the organism: Answer - mRNA
For our microarray project, the model organism under study is: Answer -
Arabidopsis thaliana
How is the protective group removed to allow the addition of nucleotides to
the probe on the DNA chip? Answer - UV light exposure
What is in a probe pair on an Affymetrix chip? Answer - One probe to the
specified gene and one probe with a mismatch in the center of the probe to
gauge non-specific hybridization levels
In the yeast microarray example, which genes are downregulated when the
yeast are grown on minimal media instead of rich media? Answer - c, d
Why are side groups on a nucleotide capped when building probes on a
microarray? Answer - To prevent elongation of probes that have incorporated
a nucleotide in the wrong place
, What plant phytohormone inhibits seed germination to prevent premature
sprouting? Answer - Abscisic acid
In which processes is sugar signaling involved? Answer - Starch metabolism,
Flowering, Heat senescence, Seed germination
If sugar levels in a plant are high, you would expect expression of genes
involved in photosynthesis to be: Answer - Downregulated
Which of the following represents a properly balanced centrifuge (check all
that apply): Answer - a
How will the plasmid DNA be separated from chromosomal DNA in this plasmid
purification lab? Answer - Differential centrifugation
What happens to plasmid DNA if you leave it in Solution II, lysis buffer, for too
long? Answer - DNA Degrades
After lysing your E. coli cells and neutralizing the reaction, do you keep the
supernatant or pellet for the next step? Answer - Supernatant
Which has more resolving power? Answer - 4% agarose gel
What should you do with the liquid waste from this lab? Answer - Bleach it for
20 minutes
Why must you wait 10 minutes after adding TE/RNase to the dry DNA pellet?
Answer - To allow time for the RNA to be hydrolyzed or degraded
