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Bio 243 Lab Final Exam – 65 Questions on DNA, Mitosis, Plasmids, Evolution

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This document contains 65 fully answered exam questions from the Bio 243 Laboratory Final Exam for the 2025/2026 academic year, offering an in-depth overview of key topics in molecular and cellular biology. It's designed for university students engaged in lab-based biological sciences and serves as a comprehensive preparation tool for practical or theoretical assessments. Covered topics span from the structure and chemical composition of DNA, including bonding, base pairing, and antiparallel arrangement, to DNA replication, packaging, and extraction techniques—such as using strawberries and detergents for efficient DNA isolation. It also details microscopy-based observations of mitosis in onion root tips, with clear references to experimental controls, dye usage, and phase identification. The document extensively addresses meiosis and its genetic significance, including segregation, independent assortment, bivalent formation, and allele behavior in Drosophila mutants. Students will also find well-explained sections on plasmid-based genetic engineering, including the pGLO system, GFP expression, heat-shock transformation, and antibiotic resistance mechanisms. Statistical analysis is well-represented through explanations of the Chi-square test, t-test, SEM, and Hardy-Weinberg Equilibrium. Additionally, it touches on natural selection, mutation types (silent, missense, nonsense), UV-induced DNA damage, and the impact of salinity gradients on bacterial growth. This study document is ideal for students enrolled in: BSc Biology Biomedical Sciences Genetics and Molecular Biology Pre-Medical Programs Life Sciences and Biotechnology Bioengineering or Evolutionary Biology Tracks Its thorough explanations, correct answers, and applied context make it a highly effective tool for mastering lab exam content, particularly at the university level. Keywords: DNA structure, DNA replication, DNA extraction, mitosis, meiosis, plasmid transformation, pGLO lab, GFP expression, genetic engineering, bacterial transformation, allele frequency, Hardy-Weinberg equilibrium, t-test, chi-square test, mutation types, UV DNA damage, natural selection, lab exam biology, population genetics, university biology

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Bio 243 Lab Final Exam 2025/2026 Exam
Questions with 100% Correct Answers |
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What is DNA? - 🧠 ANSWER ✔✔DNA is a biomolecule which contains the

genetic blueprint for all living organisms.


What is the DNA chain made of? What are the parts? - 🧠 ANSWER ✔✔The

DNA chain is made of deoxyribonucleotides. The three parts are N-bases,

sugar, and phosphate group(s).

Name the pyrimidines. How many ring structures do they have? - 🧠

ANSWER ✔✔Thymine and Cytosine. They are single ring structures.


Name the purines. How many ring structures do they have? - 🧠 ANSWER

✔✔Adenine and Guanine. They are double ring structures.

,How many hydrogen bonds does adenine and thymine have? - 🧠 ANSWER

✔✔Two


How many hydrogen bonds does cytosine and guanine have? - 🧠 ANSWER

✔✔Three


Through which bond are the P-group and the N-base bonded to the sugar?

and on which carbon? - 🧠 ANSWER ✔✔Through covalent bonding. For the

N-base, it bonds onto the C-1' and for the P- group, it bonds onto the C-5'.

Which bond joins the two strands of DNA together? - 🧠 ANSWER

✔✔Hydrogen bonds


What does one nucleotide consist of? - 🧠 ANSWER ✔✔A sugar, one base,

and a phosphate group.

What is the phosphodiester bond? Characteristics? Direction? - 🧠

ANSWER ✔✔It is when deoxyribonucleotides get joined together through

covalent bonds. It goes from 5'-3', and you add new nucleotides to the 3'

end of the previous nucleotide present in the DNA chain. It is important for

DNA replication to start, and is catalyzed by DNA polymerases.

, What is the external structures of the DNA responsible for? - 🧠 ANSWER

✔✔DNA synthesis, gives a framework for supporting the overall structure of

the DNA molecule.

What is the internal structures of the DNA responsible for? - 🧠 ANSWER

✔✔Storage of genetic information in the N bases, central dogma.


What does the DNA polymerase do? - 🧠 ANSWER ✔✔Takes a single

nucleotide from the template strand and creates complementary

nucleotides.


How is the antiparallel nature of DNA made? - 🧠 ANSWER ✔✔It is a result

of the hydrogen bonding interactions between the nitrogen bases. It occurs

when a base has to pair itself with another base at the 3' end, which is

opposite.


What is the anti conformation of DNA? - 🧠 ANSWER ✔✔When the rotation

around the covalent bond between the nitrogen base and sugar cause the

nitrogen base to lie flat, instead of being upright.


How does DNA fit into a nucleus in eukaryotes? - 🧠 ANSWER ✔✔It wounds

itself around positively charged histone proteins and helps fold DNA into

chromatin.

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