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ASCP MLS 2025 Exam with Verified Solutions – Comprehensive Practice Questions and Explanations for Medical Laboratory Science Certification

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This document provides a complete set of verified questions and detailed answers for the 2025 ASCP Medical Laboratory Scientist (MLS) exam. It covers essential topics across hematology, immunology, microbiology, clinical chemistry, transfusion medicine, urinalysis, and molecular diagnostics. Each question includes the correct answer and explanation, making it ideal for exam preparation and concept reinforcement. Perfect for students and professionals aiming to pass the ASCP MLS board exam with confidence.

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ASCP MLS 2025 EXAM WITH VERIFIED
SOLUTIONS.



A biomarker with a high specificity: - correct answer -accurately detects the
absence of disease and has few false-positives




Leukemoid Reaction Lab Results: - correct answer -A high white blood cell
count, usually 50-100 x109/L with a left shift is a common finding in
leukemoid reactions. In addition, a key feature of a leukemoid reaction is a
high LAP score




Low LAP score: - correct answer -Paroxysmal Nocturnal Hemoglobinuria,
CML




Tay-Sachs Disease: - correct answer -A rare inherited disorder characterized
by the near-total deficiency of the enzyme N- acetylbetahexosaminidase A.




Term that means varying degree of leukocytosis with a shift to the left and
occasional nucleated red cell in the peripheral blood: - correct answer -
Leukoerythroblastosis




Alder-Reilly inclusions: - correct answer -Mucopolysaccarids

,Sodium nitroprusside on a urine reagent strip reacts with: - correct answer -
Acetoacetic (diacetic) acid- ketones




Glucose levels whole blood, serum and CSF: - correct answer -
serum>whole blood>CSF




Increased numbers of basophils are often seen in: - correct answer -
Chronic myelocytic leukemia




HLA antibodies are: - correct answer -HLA antibodies are formed in
response to pregnancy, transfusion or transplantation and are therefore not
naturally occurring. They are associated with febrile nonhemolytic
transfusion reactions and TRALI. They are directed against antigens found
on granulocytes and other cells such as platelets.



Which of the following combinations is useful for confirming the presence
of extended spectrum beta-lactamases in E coli? - correct answer -Extended
spectrum beta lactamases (ESBL) are inhibited by clavulanic acid.
Confirmatory tests of the presence of ESBL are based on the enhanced
activity of a beta-lactam antibiotic, usually cefotaxime or ceftazidime, when
it is tested with clavulanic acid compared to the activity of the betalactam
tested alone.




Low PCO2: - correct answer -Represents alkalosis

, Low HCO3: - correct answer -Represents acidosis




Seriological marker for:
Rheumatic fever
Wegener's
granulomatosis CREST
syndrome
Primary biliary cirrhosis - correct answer -Antimyocardial antibodies
Antineutrophili antibodies
Anticentromere antibodies
Antimitochondrial antibodies




Which T cell expresses the CD8 marker and acts specifially to kill tumor or
virally infected cells? - correct answer -T cytotoxic




The most frequent genotype among Rho (D)- negative persons is:
- correct answer -rr




Approximately what percent of the overall population is Rh negative: -
correct answer -15%




Chylomicrons are primarily composed of: - correct answer - Triglycerides

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