METHODS IN MOLECULAR BIOLOGY STUDY GUIDE QUESTIONS AND ANSWERS 2025/2026

METHODS IN MOLECULAR BIOLOGY STUDY GUIDE QUESTIONS AND ANSWERS 2025/2026 There are 2 types of nucleic acid substitutions. Name and describe each one. - ANS-1. Transitions: substitution of a purine from another purine base or pyrimidine from another pyrimidine base (C ↔T or A↔ G) 2. Transversions: substitution of a purine from a pyrimidine or pyrimidine from a purine the genetic code is read in triplet nucleotides called ___ - ANS-codons Each codon corresponds to one ___ ___ - ANS-amino acid an open reading frame is a continuous DNA sequence that begins with a __ codon and ends with a __ codon; a length that is a multiple of __ nucleotides in between - ANS-start, stop; 3 How many reading frames for double helix DNA? How is it split? - ANS-6; 3 reading frames on top strand, 3 reading frames on bottom strand 3 types of nucleotide substitution: 1. __ mutation: same amino acid, no effect on protein sequence, often the 3rd base 2. __ mutation: amino acid substitution (i.e. GAG