QUESTIONS AND CORRECT DETAILED ANSWERS (VERIFIED
ANSWERS) |ALREADY GRADED A+||BRAND NEW!!
A nanometer is defined as: - CORRECT ANSWER-10 -9 or one-billionth of a meter
True or False: A nanometer is longer than a micrometer - CORRECT ANSWER-False
A nanometer is 1000x smaller than a micrometer
Resolution and contrast are two critical factors that influence your ability to see
an object. Explain each. - CORRECT ANSWER-Resolution refers to the distance
between two objects at which the objects can still be seen as separate. Poor or
low resolution means two or more objects may appear as one.
Contrast on the other hand is the difference in light absorbance between two
objects. Poor contrast gives a high background and makes the visualization of
multiple objects difficult. For instance, trying to identify 2 dark colored objects at
night (low light = low contrast) versus the same 2 objects in the middle of
a sunny afternoon (bright light against 2 dark objects = high contrast).
, Assuming a fixed ocular, identify the part of the microscope you would adjust to
enhance the magnification of a sample. - CORRECT ANSWER-Objective
Only the oculars (eyepiece) and the objectives contribute to the magnification of
the sample. Since the eyepiece
is fixed, only the objectives could be altered.
Assuming a constant (non-adjustable) light source power, identify the part of the
microscope you would adjust to limit the amount of light entering the
microscope. - CORRECT ANSWER-Iris diaphragm
What is the total magnification on (relative to your eye) of a sample imaged with
a 60x objective and a 10x eyepiece? Show your math. - CORRECT ANSWER-60x
objective x 10x ocular = 600x magnification
True or False: Staining is often required to image a cell that is adherent and flat
(thin). - CORRECT ANSWER-True
Adherent, flat and unstained cells are almost invisible due to the limits on both
resolution and contrast. Therefore, cell staining is often required to adequately
image
the sample.