IUPUI Cell Biology Exam #2: Complete Q&A with Verified Solutions 2025/2026

particular type of cell as a template. complementary DNA (cDNA) - DNA molecule synthesized from an mRNA molecule and therefore lacking the introns that are present in genomic DNA. dideoxy sequencing or Sanger sequencing - The standard method of determining the nucleotide sequence of DNA; utilizes DNA polymerase and a set of chain-terminating nucleotides. DNA cloning - Production of many identical copies of a DNA sequence; can be made by forcing DNA to copy sequences DNA library - Collection of cloned DNA molecules, representing either an entire genome (genomic library) or copies of the mRNA produced by a cell (cDNA library). DNA microarray - cDNA are made from samples and labeled with fluorescent dyes; expression of different mRNAs of each sample is monitored by examining the intensity and color of the fluorescent signals