-Genetic_ Engineering
Direct
·
manipulation of
genes
Transgene
·
is put into other
gene organism
Recombinent
·
to GMO
DNA
technology moves
genes create a
·
Recombinent _
DNA-
·
Uses manipulated bacteria (E Coli) .
Quick to
-
reproduce
Easy to
-
culture
Has plasmid bacteria
·
plasmid accepted by all
#
·
Plasmid is removed and cut restriction enzyme
Human
·
insulin
gene
:
Taken from
-
Pancreas
Removed cut
-
and
Insulin bacteria plasmid
·
added to
gene
DNA fuses plasmid
ligase
·
and human
gene
Makes recombinent plasmid
Not
·
all plasmids take gene! up (non-recombinent clasmid)
resistant "trailer
3)
Anti-biotic
·
added to
gene gene
Some bacteria plasmids already anti-biotic resistant !
·
Recombinent + non-recombinent plasmid mixed with E . Coli
Introduces plasmid to host
-
cells
Only
·
some E Coli pick plasmids (R + non-R)
.
up
These are transformed bacteria
Bacteria
·
to
agar plate
added with ampicilin
Only transformed bacteria
·
survives!
Anti-biotics bacteria
-
added to
Only bacteria with human + trailer
gene survive
Bacteria
·
with human placed in fermentation tank
gene
-Multiplies
-
Produces insulin
Direct
·
manipulation of
genes
Transgene
·
is put into other
gene organism
Recombinent
·
to GMO
DNA
technology moves
genes create a
·
Recombinent _
DNA-
·
Uses manipulated bacteria (E Coli) .
Quick to
-
reproduce
Easy to
-
culture
Has plasmid bacteria
·
plasmid accepted by all
#
·
Plasmid is removed and cut restriction enzyme
Human
·
insulin
gene
:
Taken from
-
Pancreas
Removed cut
-
and
Insulin bacteria plasmid
·
added to
gene
DNA fuses plasmid
ligase
·
and human
gene
Makes recombinent plasmid
Not
·
all plasmids take gene! up (non-recombinent clasmid)
resistant "trailer
3)
Anti-biotic
·
added to
gene gene
Some bacteria plasmids already anti-biotic resistant !
·
Recombinent + non-recombinent plasmid mixed with E . Coli
Introduces plasmid to host
-
cells
Only
·
some E Coli pick plasmids (R + non-R)
.
up
These are transformed bacteria
Bacteria
·
to
agar plate
added with ampicilin
Only transformed bacteria
·
survives!
Anti-biotics bacteria
-
added to
Only bacteria with human + trailer
gene survive
Bacteria
·
with human placed in fermentation tank
gene
-Multiplies
-
Produces insulin
