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Phlebotomy - Process of collecting blood
Venipuncture - The process of collecting or "drawing" blood from a vein
Vacutainer - Tube that allows the drawing of multiple blood samples
Tourniquet - Device applied or tied around patient's arm to restrict blood flow, Should
restrict venous flow to inflate veins, but not arterial flow, Most common type: stretchable
disposable straps. Apply tourniquet snugly 3 to 4 in. above intended site, Never apply over open
sore, Apply over a dry washcloth or gauze if patient has sensitive skin, Ask patient to make a fist
Cephalic vein -
Basilic vein -
Antecubital - Preferred venipuncture site
Dorsal venous network -
Plasma - Liquid part of blood
serum - clear, watery fluid portion of the blood that remains after a clot has formed
biohazardous - items contaminated with blood, body fluids, secretions, or excretions
Universal precautions - Guidelines based on treating all human blood and body fluids as
potentially infectious
Standard precautions - precautions intended to minimize risk of infection transmission
when caring for all patients.
Rules developed by the CDC
PPE (Personal protection equipment) - Mask, goggles, gown, gloves
Whole blood - Fluid (plasma) and cellular (RBCs, WBCs, and platelets) components
Syringe - a medical instrument used to inject or withdraw fluids
1- to 1.5-in. long, Must have resheathing feature if used to draw blood, come in Barrel and
Plunger
, Antiseptic - used to clean site to avoid infection or contamination
OSHA (Occupational Safety and Health Administration) - a government agency in the
Department of Labor to maintain a safe and healthy work environment
CDC (Center for Disease Control) - Protect public health-safety through control and
prevention of disease
Hemoconcentration - A decrease in fluid content of blood and an increase in
nonfilterable large molecules
Caused by stagnation of normal venous flow due to tourniquet
Hemolysis - Damage to or destruction of RBCs
Hemoglobin escapes into fluid part of specimen
Anticoagulant - Substances that prevent blood from clotting. Two methods of preventing
clotting: Precipitating calcium & Inhibiting formation of thrombin. Types:
Ethylenediaminetetraacetic acid (EDTA), Citrates, Heparin, Oxalates
Needle gauge - Gauge=Diameter; Smaller gauge=fatter needle
Appropriate range for phlebotomy: 20 to 23 (21 is most common)
Order of draw - 1. Sterile tube (blood culture)
2. Blue-top coagulation tube
3. Serum tube w. or w/o. clot activator or gel
4. Heparin tube w. or w/o. gel plasma separator
5. EDTA tube
6. Glycolytic inhibitor tube
Additive - substance added to tube: Anticoagulants, special use anticoagulants,
antiglycolitic agents, clot activators, thixotropic gel separator, trace element-free tubes
Palpate - Using your finger to fine the vein on pts dominant arm (preferably) with index
finger