ACS biochemistry question with verified answers
an example of a blunt restriction enzyme? Ans✓✓✓ ACG TGA
an example of a sticky restriction enzyme? Ans✓✓✓ A CGTGA
Based on the mechanism of alkaline hydrolysis of RNA, why is DNA less
susceptible to alkaline hydrolysis? Ans✓✓✓ DNA lacks the 2' hydroxyl group
Describe the structural changes in hemoglobin that allow for cooperative binding
of O2 Ans✓✓✓ Binding of O2 in one subunit causes a conformational change in
an adjacent subunit allowing O2 to bind
Describe what metabolic processes occur in the muscle and liver in the glucose-
alanine cyvles during branched chain amino acid catabolism Ans✓✓✓ branched
chain amino acids are degraded in the muscle and the NH3 is transported to the
liver in the form of Ala. The liver uses the Ala for gluconeogenesis and the glucose
is transported to the muscle for glycolysis.
Does myoglobin show cooperativity in O2 binding? If not, what structural
differences account for this? Ans✓✓✓ Myoglobin does not show cooperativity
because it is monomeric
From a practical standpoint, would it be advantageous for these potential drugs
to be less chemically stable than the substrate? Ans✓✓✓ No, because of shelf
life and time to reach target
How are beta sheets stabilized in proteins? Ans✓✓✓ Hydrogen bonding between
peptide backbone groups
, If triglycerides and phospholipids are separated using TLC, what spot is more
soluble (will be at the top)? Ans✓✓✓ tryglyceride; phospholipid will be less
soluble so will not travel as far
In a gel filtration column, what proteins elute the quickest? Ans✓✓✓ the larger
proteins that have a higher kDa elute first
In DNA, which bases hydrogen bond? Ans✓✓✓ A-T; G-C
In each base pair, how many hydrogen bonds are there? Ans✓✓✓ A-T=2; G-C=3
Is transverse or lateral diffusion in a lipid bilayer least likely to occur? Ans✓✓✓
transverse
List all the enzymes that use OAA as a substrate Ans✓✓✓ Pyruvate carboxylase,
citrate synthase, malate dehydrogenase
name an allosteric regulator that can decrease glycolysis by inhibiting PFK
Ans✓✓✓ ATP
Name three secondary messengers and what class of biomolecules they are
derived from Ans✓✓✓ cAMP-nucleotide; IP3-lipid; DAG-lipid
what amino acid sequence is most likely to be in the core of a globular protein?
why? Ans✓✓✓ ITFWLI; no charged amino acids and highest percent nonpolar
an example of a blunt restriction enzyme? Ans✓✓✓ ACG TGA
an example of a sticky restriction enzyme? Ans✓✓✓ A CGTGA
Based on the mechanism of alkaline hydrolysis of RNA, why is DNA less
susceptible to alkaline hydrolysis? Ans✓✓✓ DNA lacks the 2' hydroxyl group
Describe the structural changes in hemoglobin that allow for cooperative binding
of O2 Ans✓✓✓ Binding of O2 in one subunit causes a conformational change in
an adjacent subunit allowing O2 to bind
Describe what metabolic processes occur in the muscle and liver in the glucose-
alanine cyvles during branched chain amino acid catabolism Ans✓✓✓ branched
chain amino acids are degraded in the muscle and the NH3 is transported to the
liver in the form of Ala. The liver uses the Ala for gluconeogenesis and the glucose
is transported to the muscle for glycolysis.
Does myoglobin show cooperativity in O2 binding? If not, what structural
differences account for this? Ans✓✓✓ Myoglobin does not show cooperativity
because it is monomeric
From a practical standpoint, would it be advantageous for these potential drugs
to be less chemically stable than the substrate? Ans✓✓✓ No, because of shelf
life and time to reach target
How are beta sheets stabilized in proteins? Ans✓✓✓ Hydrogen bonding between
peptide backbone groups
, If triglycerides and phospholipids are separated using TLC, what spot is more
soluble (will be at the top)? Ans✓✓✓ tryglyceride; phospholipid will be less
soluble so will not travel as far
In a gel filtration column, what proteins elute the quickest? Ans✓✓✓ the larger
proteins that have a higher kDa elute first
In DNA, which bases hydrogen bond? Ans✓✓✓ A-T; G-C
In each base pair, how many hydrogen bonds are there? Ans✓✓✓ A-T=2; G-C=3
Is transverse or lateral diffusion in a lipid bilayer least likely to occur? Ans✓✓✓
transverse
List all the enzymes that use OAA as a substrate Ans✓✓✓ Pyruvate carboxylase,
citrate synthase, malate dehydrogenase
name an allosteric regulator that can decrease glycolysis by inhibiting PFK
Ans✓✓✓ ATP
Name three secondary messengers and what class of biomolecules they are
derived from Ans✓✓✓ cAMP-nucleotide; IP3-lipid; DAG-lipid
what amino acid sequence is most likely to be in the core of a globular protein?
why? Ans✓✓✓ ITFWLI; no charged amino acids and highest percent nonpolar
