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Microbiology Test Bank Study Questions and Answers (2025 Graded A+)

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Penicillin controls bacterial growth by: A) preventing synthesis of the lipopolysaccharide layer B) inhibiting protein synthesis C) preventing cross-link formation in peptidoglycan D) preventing generation of the proton motive force - Correct Ans-preventing cross-link formation in peptidoglycan Reproduction via binary fission leads to what type of growth under optimal conditions: A) linear B) mixed C) exponential D) uptake-limited - Correct Ans-exponential Protection against osmotic lyses is provided by: A) lysosomes B) the cellular plasma membrane C) the cell's capsule D) the cell wall - Correct Ans-the cell wall Name associated with the presense of a cell wall and a capsule together in a bacterial cell wall: A) the envelope B) the capsule C) the cytoplasmic membrane D) the cell wall - Correct Ans-the envelope Which of the following is true about the lag phase: A) cells adapt to a new medium B) in some cases it may be very short C) cells replenish spent materials D) all of the above - Correct Ans-all of the above A student is asked by the professor to sub-culture a bacterial organism onto fresh media for future studies. However during sub-culturing the students lock the test tube preventing oxygen flow into the media. After days the culture dyes. What type of culture did the student have at hand: A) obligate aerobe B) strict anaerobe C) facultative anaerobe D) none of the above - Correct Ans-obligate aerobe Staining procedure designed for bacterial families with high lipid content and resistant to standard staining: A) gram stain B) negative staining C) acid fast staining D) simple stain - Correct Ans-acid fast staining Which of the following is not considered a starvation response? A) endospore formation B) synthesis of starvation proteins C) cell death at a logrtihmic rate D) a decrease in cellular size and nucleoid condensation - Correct Ans-cell death at a logrtihmic rate Organelle responsible for the synthesis of protein in a eukaryotic cell: A) the smooth ER B) the rough ER C) the Golgi complex D) the nucleus - Correct Ans-the rough ER Which of the following is the most important structure related to microbial attachment to cells? A) flagellum B) plasmid C) peptidoglycan D) glycocalix - Correct Ans-glycocalix Which of the following statement(s) is/are correct when comparing prokaryotic and eukaryotic genome? A) bacteria mostly have one circular DNA molecule B) Eukaryotes have linear DNA C) Both A and B D) None of the above - Correct Ans-Both A and B ___________ membranes allow some molecules to pass but not all. A) Permeable B) Inverted Membrane C) Selectively Membrane D) Impermeable - Correct Ans-Selectively Membrane The ribosome binding site A) forms a stem-loop structure in the RNA B) is located upstream of the promoter sequence C) is located immediately upstream of the start codon D) is more likely to be associated with an operon than with a gene encoding a single protein - Correct Ans-is located immediately upstream of the start codon The nucleus of a eukaryote does not contain A) nuclear membrane B) nucleoid C) nuclear pores D) nucleolus - Correct Ans-nucleoid The protein coat and lipid envelopes are components that help to protect the viral A) capsid B) genome C) tegument D) spikes - Correct Ans-genome Which of the following helps protect bacteria from lysis in dilute solution and helps to determine cellular morphology? A) plasma membrane B) capsule C) gas vacuoles D) cell wall - Correct Ans-cell wall

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Microbiology Test Bank



Microbiology Test Bank Study Questions
and Answers (2025 Graded A+)
Penicillin controls bacterial growth by:
A) preventing synthesis of the lipopolysaccharide layer
B) inhibiting protein synthesis
C) preventing cross-link formation in peptidoglycan
D) preventing generation of the proton motive force - Correct Ans-preventing cross-link
formation in peptidoglycan


Reproduction via binary fission leads to what type of growth under optimal conditions:
A) linear
B) mixed
C) exponential
D) uptake-limited - Correct Ans-exponential


Protection against osmotic lyses is provided by:
A) lysosomes
B) the cellular plasma membrane
C) the cell's capsule
D) the cell wall - Correct Ans-the cell wall


Name associated with the presense of a cell wall and a capsule together in a bacterial
cell wall:
A) the envelope
B) the capsule

1|Page

,Microbiology Test Bank


C) the cytoplasmic membrane D) the cell wall - Correct Ans-the envelope


Which of the following is true about the lag phase:
A) cells adapt to a new medium B) in some cases it may be very short
C) cells replenish spent materials
D) all of the above - Correct Ans-all of the above


A student is asked by the professor to sub-culture a bacterial organism onto fresh media
for future studies. However during sub-culturing the students lock the test tube
preventing oxygen flow into the media. After days the culture dyes. What type of culture
did the student have at hand:
A) obligate aerobe
B) strict anaerobe
C) facultative anaerobe
D) none of the above - Correct Ans-obligate aerobe


Staining procedure designed for bacterial families with high lipid content and resistant to
standard staining:
A) gram stain
B) negative staining
C) acid fast staining
D) simple stain - Correct Ans-acid fast staining


Which of the following is not considered a starvation response?
A) endospore formation
B) synthesis of starvation proteins



2|Page

,Microbiology Test Bank


C) cell death at a logrtihmic rate D) a decrease in cellular size and nucleoid condensation
- Correct Ans-cell death at a logrtihmic rate


Organelle responsible for the synthesis of protein in a eukaryotic cell:
A) the smooth ER
B) the rough ER
C) the Golgi complex
D) the nucleus - Correct Ans-the rough ER


Which of the following is the most important structure related to microbial attachment
to cells? A) flagellum
B) plasmid
C) peptidoglycan
D) glycocalix - Correct Ans-glycocalix


Which of the following statement(s) is/are correct when comparing prokaryotic and
eukaryotic genome?
A) bacteria mostly have one circular DNA molecule
B) Eukaryotes have linear DNA C) Both A and B
D) None of the above - Correct Ans-Both A and B


___________ membranes allow some molecules to pass but not all.
A) Permeable
B) Inverted Membrane
C) Selectively Membrane
D) Impermeable - Correct Ans-Selectively Membrane


3|Page

, Microbiology Test Bank


The ribosome binding site
A) forms a stem-loop structure in the RNA
B) is located upstream of the promoter sequence
C) is located immediately upstream of the start codon
D) is more likely to be associated with an operon than with a gene encoding a single
protein - Correct Ans-is located immediately upstream of the start codon


The nucleus of a eukaryote does not contain
A) nuclear membrane
B) nucleoid
C) nuclear pores
D) nucleolus - Correct Ans-nucleoid


The protein coat and lipid envelopes are components that help to protect the viral
A) capsid
B) genome
C) tegument
D) spikes - Correct Ans-genome


Which of the following helps protect bacteria from lysis in dilute solution and helps to
determine cellular morphology?
A) plasma membrane
B) capsule
C) gas vacuoles
D) cell wall - Correct Ans-cell wall




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